Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster

Shaun Leivers, Claudio Hidalgo-cantabrana, Glenn Robinson, Abelardo Margolles, Patricia Ruas-madiedo, Andrew P. Laws

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The bile adapted strain Bifidobacterium animalis subsp. lactis IPLA-R1 secretes a high molecular weight exopolysaccharide (HMW-EPS) when grown on the surface of agar-MRSC. This EPS is composed of l-rhamnopyranosyl, d-glucopyranosyl, d-galactopyranosyl and d-galactofuranosyl residues in the ratio of 3:1:1:1. Linkage analysis and 1D and 2D NMR spectroscopy were used to show that the EPS has a hexasaccharide repeating unit with the following structure:

Treatment of the EPS with mild acid cleanly removed the terminal d-galactofuranosyl residue. The eps cluster sequenced for strain IPLA-R1 showed high genetic homology with putative eps clusters annotated in the genomes of strains from the same species. It is of note that several genes coding for rhamnose-precursors are present in the eps cluster, which could be correlated with the high percentage of rhamnose detected in its EPS repeated unit.
Original languageEnglish
Pages (from-to)2710-2717
JournalCarbohydrate Research
Volume346
Issue number17
Early online date22 Sep 2011
DOIs
Publication statusPublished - 13 Dec 2011

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Rhamnose
Sequence Analysis
Molecular Weight
Molecular weight
Genes
Bile
Agar
Magnetic Resonance Spectroscopy
Genome
Nuclear magnetic resonance spectroscopy
Acids
Bifidobacterium animalis

Cite this

Leivers, Shaun ; Hidalgo-cantabrana, Claudio ; Robinson, Glenn ; Margolles, Abelardo ; Ruas-madiedo, Patricia ; Laws, Andrew P. / Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster. In: Carbohydrate Research. 2011 ; Vol. 346, No. 17. pp. 2710-2717.
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Structure of the high molecular weight exopolysaccharide produced by Bifidobacterium animalis subsp. lactis IPLA-R1 and sequence analysis of its putative eps cluster. / Leivers, Shaun; Hidalgo-cantabrana, Claudio; Robinson, Glenn; Margolles, Abelardo; Ruas-madiedo, Patricia; Laws, Andrew P.

In: Carbohydrate Research, Vol. 346, No. 17, 13.12.2011, p. 2710-2717.

Research output: Contribution to journalArticle

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AU - Laws, Andrew P.

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