Structures of the Ets protein DNA-binding domains of transcription factors Etv1, Etv4, Etv5, and Fev: Determinants of DNA binding and redox regulation by disulfide bond formation

Christopher D O Cooper, Joseph A. Newman, Hazel Aitkenhead, Charles K. Allerston, Opher Gileadi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Ets transcription factors, which share the conserved Ets DNA-binding domain, number nearly 30 members in humans and are particularly involved in developmental processes. Their deregulation following changes in expression, transcriptional activity, or by chromosomal translocation plays a critical role in carcinogenesis. Ets DNA binding, selectivity, and regulation have been extensively studied; however, questions still arise regarding binding specificity outside the core GGA recognition sequence and the mode of action of Ets post-translational modifications. Here, we report the crystal structures of Etv1, Etv4, Etv5, and Fev, alone and in complex with DNA. We identify previously unrecognized features of the protein-DNA interface. Interactions with the DNA backbone account for most of the binding affinity. We describe a highly coordinated network of water molecules acting in base selection upstream of the GGAA core and the structural features that may account for discrimination against methylated cytidine residues. Unexpectedly, all proteins crystallized as disulfide-linked dimers, exhibiting a novel interface (distant to the DNA recognition helix). Homodimers of Etv1, Etv4, and Etv5 could be reduced to monomers, leading to a 40-200-fold increase in DNA binding affinity. Hence, we present the first indication of a redox-dependent regulatory mechanism that may control the activity of this subset of oncogenic Ets transcription factors.

LanguageEnglish
Pages13692-13709
Number of pages18
JournalJournal of Biological Chemistry
Volume290
Issue number22
DOIs
Publication statusPublished - 29 May 2015
Externally publishedYes

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DNA-Binding Proteins
Disulfides
Oxidation-Reduction
Transcription Factors
DNA
Proto-Oncogene Proteins c-ets
Cytidine
Genetic Translocation
Deregulation
Post Translational Protein Processing
Dimers
Carcinogenesis
Proteins
Monomers
Crystal structure
Molecules
Water

Cite this

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title = "Structures of the Ets protein DNA-binding domains of transcription factors Etv1, Etv4, Etv5, and Fev: Determinants of DNA binding and redox regulation by disulfide bond formation",
abstract = "Ets transcription factors, which share the conserved Ets DNA-binding domain, number nearly 30 members in humans and are particularly involved in developmental processes. Their deregulation following changes in expression, transcriptional activity, or by chromosomal translocation plays a critical role in carcinogenesis. Ets DNA binding, selectivity, and regulation have been extensively studied; however, questions still arise regarding binding specificity outside the core GGA recognition sequence and the mode of action of Ets post-translational modifications. Here, we report the crystal structures of Etv1, Etv4, Etv5, and Fev, alone and in complex with DNA. We identify previously unrecognized features of the protein-DNA interface. Interactions with the DNA backbone account for most of the binding affinity. We describe a highly coordinated network of water molecules acting in base selection upstream of the GGAA core and the structural features that may account for discrimination against methylated cytidine residues. Unexpectedly, all proteins crystallized as disulfide-linked dimers, exhibiting a novel interface (distant to the DNA recognition helix). Homodimers of Etv1, Etv4, and Etv5 could be reduced to monomers, leading to a 40-200-fold increase in DNA binding affinity. Hence, we present the first indication of a redox-dependent regulatory mechanism that may control the activity of this subset of oncogenic Ets transcription factors.",
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Structures of the Ets protein DNA-binding domains of transcription factors Etv1, Etv4, Etv5, and Fev : Determinants of DNA binding and redox regulation by disulfide bond formation. / Cooper, Christopher D O; Newman, Joseph A.; Aitkenhead, Hazel; Allerston, Charles K.; Gileadi, Opher.

In: Journal of Biological Chemistry, Vol. 290, No. 22, 29.05.2015, p. 13692-13709.

Research output: Contribution to journalArticle

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