Study on Pulse Electric Field Exposure Effect on HeLa Cells for Wound Healing Application

Muhammad Mahadi Abdul Jamil, Mohamed A.Milad Zaltum, Mansour Youseffi, Farideh Javid

Research output: Contribution to journalConference article

Abstract

This study investigated the effect of pulse electric field (PEF) exposure on cervical cancer cells (HeLa cells) in an in-vitro wound repair model. The study mainly focused on the healing time of HeLa cell line wound model. During the experiments HeLa cells were maintained at 37?C in a modified Chamlide EC magnetic chamber where they were exposed to high electric fields. A Nikon inverted microscope (Ti-series) with Metamorph® time lapse software were used to monitor, image and capture photomicrographs and videos of the cells. The tests carried out during this study revealed that pulse electric field enhanced the migration of HeLa cells. Cells exposed to PEF (1kV/cm, 100μs, and single pulse) healed the wound in ∼2 hours (from initial wound gap of 54.53μm ? 0.55SD to 0.66μm ? 0.61SD). On the other hand, non-PEF (control) healed the wound in ∼10 hours (from initial wound gap of 56.33μm ? 0.57SD to 0.46μm ? 0.45SD). It was therefore found that the healing rate with PEF is ∼five times faster than non-PEF group. It is believed that PEF usage on diseased biological cells would enable a novel method for assisting drug free wound repair systems and many other potential biomedical engineering applications such as treatment of neurological disorders including Alzheimers and Parkinsons.

Original languageEnglish
Article number012021
Number of pages8
JournalJournal of Physics: Conference Series
Volume1372
Issue number1
Early online date22 Nov 2019
DOIs
Publication statusPublished - 22 Nov 2019
Event2019 International Conference on Biomedical Engineering - Penang Island, Malaysia
Duration: 26 Aug 201927 Aug 2019
https://icobe.unimap.edu.my/

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