Synthesis, structure and bonding modes of pyrazine based ligands of Cp*Rh and Cp*Ir complexes: The study of in-vitro cytotoxicity against human cell lines

Agreeda Lapasam, Emma Pinder, Roger M. Phillips, Werner Kaminsky, Mohan Rao Kollipara

Research output: Contribution to journalArticle

Abstract

The reaction of multidentate pyrazine based ligands was explored towards Cp*rhodium and Cp*iridium precursors. Mononuclear and dinuclear complexes formed by the ratio-based reaction between ligand and metal precursor. The representative complexes have been determined by single crystal X-ray diffraction studies. Cytotoxicity study of the ligands and their complexes are evaluated against human colorectal cancer cell lines HT-29, HCT-116 p53+/+ and HCT-116 p53−/− and ARPE-19 (non-cancer retinal epithelium) cells. Complexes 2-5 and 7-8 were cytotoxic to cells and although the potency of these complexes was less than cisplatin, selectivity towards cancer cell lines as opposed to non-cancer ARPE-19 cells was comparable to cisplatin. From in-vitro cytotoxicity studies complexes 4 and 5 demonstrated good selectivity towards HCT116 p53−/− cells suggesting that these complexes are promising leads for the treatment of p53 deficient cancers.
LanguageEnglish
Article number120887
JournalJournal of Organometallic Chemistry
Volume899
Early online date8 Aug 2019
DOIs
Publication statusE-pub ahead of print - 8 Aug 2019

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Pyrazines
pyrazines
Cytotoxicity
cultured cells
Ligands
Cells
Cell Line
Cisplatin
ligands
cancer
synthesis
cells
HCT116 Cells
Iridium
Rhodium
selectivity
X-Ray Diffraction
epithelium
Colorectal Neoplasms
Neoplasms

Cite this

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title = "Synthesis, structure and bonding modes of pyrazine based ligands of Cp*Rh and Cp*Ir complexes: The study of in-vitro cytotoxicity against human cell lines",
abstract = "The reaction of multidentate pyrazine based ligands was explored towards Cp*rhodium and Cp*iridium precursors. Mononuclear and dinuclear complexes formed by the ratio-based reaction between ligand and metal precursor. The representative complexes have been determined by single crystal X-ray diffraction studies. Cytotoxicity study of the ligands and their complexes are evaluated against human colorectal cancer cell lines HT-29, HCT-116 p53+/+ and HCT-116 p53−/− and ARPE-19 (non-cancer retinal epithelium) cells. Complexes 2-5 and 7-8 were cytotoxic to cells and although the potency of these complexes was less than cisplatin, selectivity towards cancer cell lines as opposed to non-cancer ARPE-19 cells was comparable to cisplatin. From in-vitro cytotoxicity studies complexes 4 and 5 demonstrated good selectivity towards HCT116 p53−/− cells suggesting that these complexes are promising leads for the treatment of p53 deficient cancers.",
keywords = "Rhodium, Iridium, Pyrazine, In vitro cytotoxicity",
author = "Agreeda Lapasam and Emma Pinder and Phillips, {Roger M.} and Werner Kaminsky and Kollipara, {Mohan Rao}",
year = "2019",
month = "8",
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doi = "10.1016/j.jorganchem.2019.120887",
language = "English",
volume = "899",
journal = "Journal of Organometallic Chemistry",
issn = "0022-328X",
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Synthesis, structure and bonding modes of pyrazine based ligands of Cp*Rh and Cp*Ir complexes : The study of in-vitro cytotoxicity against human cell lines. / Lapasam, Agreeda; Pinder, Emma; Phillips, Roger M.; Kaminsky, Werner; Kollipara, Mohan Rao.

In: Journal of Organometallic Chemistry, Vol. 899, 120887, 30.10.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Synthesis, structure and bonding modes of pyrazine based ligands of Cp*Rh and Cp*Ir complexes

T2 - Journal of Organometallic Chemistry

AU - Lapasam, Agreeda

AU - Pinder, Emma

AU - Phillips, Roger M.

AU - Kaminsky, Werner

AU - Kollipara, Mohan Rao

PY - 2019/8/8

Y1 - 2019/8/8

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AB - The reaction of multidentate pyrazine based ligands was explored towards Cp*rhodium and Cp*iridium precursors. Mononuclear and dinuclear complexes formed by the ratio-based reaction between ligand and metal precursor. The representative complexes have been determined by single crystal X-ray diffraction studies. Cytotoxicity study of the ligands and their complexes are evaluated against human colorectal cancer cell lines HT-29, HCT-116 p53+/+ and HCT-116 p53−/− and ARPE-19 (non-cancer retinal epithelium) cells. Complexes 2-5 and 7-8 were cytotoxic to cells and although the potency of these complexes was less than cisplatin, selectivity towards cancer cell lines as opposed to non-cancer ARPE-19 cells was comparable to cisplatin. From in-vitro cytotoxicity studies complexes 4 and 5 demonstrated good selectivity towards HCT116 p53−/− cells suggesting that these complexes are promising leads for the treatment of p53 deficient cancers.

KW - Rhodium

KW - Iridium

KW - Pyrazine

KW - In vitro cytotoxicity

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