The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility

Gabriele Stakaityte, Nnenna Nwogu, Jonathan D. Lippiat, G. Eric Blair, Krzysztof Poterlowicz, James R. Boyne, Andrew MacDonald, Jamel Mankouri, Adrian Whitehouse

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ion channels regulate many aspects of cell physiology, including cell proliferation, motility, and migration, and aberrant expression and activity of ion channels is associated with various stages of tumor development, with K+ and Cl- channels now being considered the most active during tumorigenesis. Accordingly, emerging in vitro and preclinical studies have revealed that pharmacological manipulation of ion channel activity offers protection against several cancers. Merkel cell polyomavirus (MCPyV) is a major cause of Merkel cell carcinoma (MCC), primarily because of the expression of two early regulatory proteins termed small and large tumor antigens (ST and LT, respectively). Several molecular mechanisms have been attributed to MCPyV-mediated cancer formation but, thus far, no studies have investigated any potential link to cellular ion channels. Here we demonstrate that Cl- channel modulation can reduce MCPyV ST-induced cell motility and invasiveness. Proteomic analysis revealed that MCPyV ST up-regulates two Cl- channels, CLIC1 and CLIC4, which when silenced, inhibit MCPyV ST-induced motility and invasiveness, implicating their function as critical to MCPyV-induced metastatic processes. Consistent with these data, we confirmed that CLIC1 and CLIC4 are up-regulated in primary MCPyV-positiveMCCpatient samples. We therefore, for the first time, implicate cellular ion channels as a key host cell factor contributing to virus-mediated cellular transformation. Given the intense interest in ion channel modulating drugs for human disease. This highlights CLIC1 and CLIC4 activity as potential targets for MCPyV-induced MCC.

LanguageEnglish
Pages4582-4590
Number of pages9
JournalJournal of Biological Chemistry
Volume293
Issue number12
Early online date8 Feb 2018
DOIs
Publication statusPublished - 23 Mar 2018
Externally publishedYes

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Merkel cell polyomavirus
Chloride Channels
Ion Channels
Viruses
Cell Movement
Merkel Cell Carcinoma
Physiology
Cell proliferation
Neoplasm Antigens
Cell Physiological Phenomena
Neoplasms
Tumors
Cells
Modulation
Proteomics
Carcinogenesis
Up-Regulation
Cell Proliferation
Pharmacology

Cite this

Stakaityte, G., Nwogu, N., Lippiat, J. D., Blair, G. E., Poterlowicz, K., Boyne, J. R., ... Whitehouse, A. (2018). The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility. Journal of Biological Chemistry, 293(12), 4582-4590. https://doi.org/10.1074/jbc.RA117.001343
Stakaityte, Gabriele ; Nwogu, Nnenna ; Lippiat, Jonathan D. ; Blair, G. Eric ; Poterlowicz, Krzysztof ; Boyne, James R. ; MacDonald, Andrew ; Mankouri, Jamel ; Whitehouse, Adrian. / The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility. In: Journal of Biological Chemistry. 2018 ; Vol. 293, No. 12. pp. 4582-4590.
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Stakaityte, G, Nwogu, N, Lippiat, JD, Blair, GE, Poterlowicz, K, Boyne, JR, MacDonald, A, Mankouri, J & Whitehouse, A 2018, 'The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility', Journal of Biological Chemistry, vol. 293, no. 12, pp. 4582-4590. https://doi.org/10.1074/jbc.RA117.001343

The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility. / Stakaityte, Gabriele; Nwogu, Nnenna; Lippiat, Jonathan D.; Blair, G. Eric; Poterlowicz, Krzysztof; Boyne, James R.; MacDonald, Andrew; Mankouri, Jamel; Whitehouse, Adrian.

In: Journal of Biological Chemistry, Vol. 293, No. 12, 23.03.2018, p. 4582-4590.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility

AU - Stakaityte, Gabriele

AU - Nwogu, Nnenna

AU - Lippiat, Jonathan D.

AU - Blair, G. Eric

AU - Poterlowicz, Krzysztof

AU - Boyne, James R.

AU - MacDonald, Andrew

AU - Mankouri, Jamel

AU - Whitehouse, Adrian

PY - 2018/3/23

Y1 - 2018/3/23

N2 - Ion channels regulate many aspects of cell physiology, including cell proliferation, motility, and migration, and aberrant expression and activity of ion channels is associated with various stages of tumor development, with K+ and Cl- channels now being considered the most active during tumorigenesis. Accordingly, emerging in vitro and preclinical studies have revealed that pharmacological manipulation of ion channel activity offers protection against several cancers. Merkel cell polyomavirus (MCPyV) is a major cause of Merkel cell carcinoma (MCC), primarily because of the expression of two early regulatory proteins termed small and large tumor antigens (ST and LT, respectively). Several molecular mechanisms have been attributed to MCPyV-mediated cancer formation but, thus far, no studies have investigated any potential link to cellular ion channels. Here we demonstrate that Cl- channel modulation can reduce MCPyV ST-induced cell motility and invasiveness. Proteomic analysis revealed that MCPyV ST up-regulates two Cl- channels, CLIC1 and CLIC4, which when silenced, inhibit MCPyV ST-induced motility and invasiveness, implicating their function as critical to MCPyV-induced metastatic processes. Consistent with these data, we confirmed that CLIC1 and CLIC4 are up-regulated in primary MCPyV-positiveMCCpatient samples. We therefore, for the first time, implicate cellular ion channels as a key host cell factor contributing to virus-mediated cellular transformation. Given the intense interest in ion channel modulating drugs for human disease. This highlights CLIC1 and CLIC4 activity as potential targets for MCPyV-induced MCC.

AB - Ion channels regulate many aspects of cell physiology, including cell proliferation, motility, and migration, and aberrant expression and activity of ion channels is associated with various stages of tumor development, with K+ and Cl- channels now being considered the most active during tumorigenesis. Accordingly, emerging in vitro and preclinical studies have revealed that pharmacological manipulation of ion channel activity offers protection against several cancers. Merkel cell polyomavirus (MCPyV) is a major cause of Merkel cell carcinoma (MCC), primarily because of the expression of two early regulatory proteins termed small and large tumor antigens (ST and LT, respectively). Several molecular mechanisms have been attributed to MCPyV-mediated cancer formation but, thus far, no studies have investigated any potential link to cellular ion channels. Here we demonstrate that Cl- channel modulation can reduce MCPyV ST-induced cell motility and invasiveness. Proteomic analysis revealed that MCPyV ST up-regulates two Cl- channels, CLIC1 and CLIC4, which when silenced, inhibit MCPyV ST-induced motility and invasiveness, implicating their function as critical to MCPyV-induced metastatic processes. Consistent with these data, we confirmed that CLIC1 and CLIC4 are up-regulated in primary MCPyV-positiveMCCpatient samples. We therefore, for the first time, implicate cellular ion channels as a key host cell factor contributing to virus-mediated cellular transformation. Given the intense interest in ion channel modulating drugs for human disease. This highlights CLIC1 and CLIC4 activity as potential targets for MCPyV-induced MCC.

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DO - 10.1074/jbc.RA117.001343

M3 - Article

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T2 - Journal of Biological Chemistry

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