The cytotoxicity effect of cannabidiol on the human stomach carcinoma cells.

Ahmed Saleh, Arin Saha, Brain Dobbins, Roger Phillips, Farideh Javid

Research output: Contribution to journalMeeting Abstractpeer-review


For gastric cancer surgery in combination with chemotherapy is the only curative option, however the treatment is highly invasive and only a relatively small proportion of patients are either fit enough for or suitable for surgery [1]. It would therefore be beneficial to determine if novel agents could play a role in the treatment of these cancers and improve outcomes for patients. There is evidence that cannabinoids induce anti-cancer properties in variety of cancers however the evidence for the non-psychoactive cannabinoid CBD in in gastric cancer is limited. The aim of the present study is to investigate if cannabidiol induces cytotoxicity in stomach carcinoma cells.
AGS gastric cancer cell lines were maintained and sub-cultured as per American Type Culture Collection (ATCC) guidelines. When cells reached70% confluence, they were seeded in 96 well plates and treated with cannabidiol at concentrations ranging from 0.2μmol to 100μmol or vehicle. After 96 hours contact time, cell viability was assessed using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Experiments were repeated independently four times. Cell viability at each concentration was recorded and half maximal inhibitory concentration (IC50)was calculated. Results were expressed as the mean+s.e.mean of N=4. Statistical analysis was used using one way ANOVA followed by a t-test,pResults
Pre-treatment with cannabidiol (0.2-100μmol) induced cytotoxicity in concentration dependent manner. The cell viability was significantly(pinvestigate if a sub-lethal dose of cannabidiol when combined with cisplatin can induce a stronger cytotoxicity compared to the application of cis-platin alone
Original languageEnglish
Article numberPOO97
Pages (from-to)439
Number of pages1
JournalBritish Journal of Pharmacology
Issue numberS1
Early online date27 Jun 2023
Publication statusPublished - 1 Jul 2023
Event19th World Congress of Basic & Clinical Pharmacology - Glasgow, United Kingdom
Duration: 2 Jul 20237 Jul 2023
Conference number: 19

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