Abstract
Introduction
For gastric cancer surgery in combination with chemotherapy is the only curative option, however the treatment is highly invasive and only a relatively small proportion of patients are either fit enough for or suitable for surgery [1]. It would therefore be beneficial to determine if novel agents could play a role in the treatment of these cancers and improve outcomes for patients. There is evidence that cannabinoids induce anti-cancer properties in variety of cancers however the evidence for the non-psychoactive cannabinoid CBD in in gastric cancer is limited. The aim of the present study is to investigate if cannabidiol induces cytotoxicity in stomach carcinoma cells.
Method
AGS gastric cancer cell lines were maintained and sub-cultured as per American Type Culture Collection (ATCC) guidelines. When cells reached70% confluence, they were seeded in 96 well plates and treated with cannabidiol at concentrations ranging from 0.2μmol to 100μmol or vehicle. After 96 hours contact time, cell viability was assessed using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Experiments were repeated independently four times. Cell viability at each concentration was recorded and half maximal inhibitory concentration (IC50)was calculated. Results were expressed as the mean+s.e.mean of N=4. Statistical analysis was used using one way ANOVA followed by a t-test,pResults
Pre-treatment with cannabidiol (0.2-100μmol) induced cytotoxicity in concentration dependent manner. The cell viability was significantly(pinvestigate if a sub-lethal dose of cannabidiol when combined with cisplatin can induce a stronger cytotoxicity compared to the application of cis-platin alone
For gastric cancer surgery in combination with chemotherapy is the only curative option, however the treatment is highly invasive and only a relatively small proportion of patients are either fit enough for or suitable for surgery [1]. It would therefore be beneficial to determine if novel agents could play a role in the treatment of these cancers and improve outcomes for patients. There is evidence that cannabinoids induce anti-cancer properties in variety of cancers however the evidence for the non-psychoactive cannabinoid CBD in in gastric cancer is limited. The aim of the present study is to investigate if cannabidiol induces cytotoxicity in stomach carcinoma cells.
Method
AGS gastric cancer cell lines were maintained and sub-cultured as per American Type Culture Collection (ATCC) guidelines. When cells reached70% confluence, they were seeded in 96 well plates and treated with cannabidiol at concentrations ranging from 0.2μmol to 100μmol or vehicle. After 96 hours contact time, cell viability was assessed using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. Experiments were repeated independently four times. Cell viability at each concentration was recorded and half maximal inhibitory concentration (IC50)was calculated. Results were expressed as the mean+s.e.mean of N=4. Statistical analysis was used using one way ANOVA followed by a t-test,pResults
Pre-treatment with cannabidiol (0.2-100μmol) induced cytotoxicity in concentration dependent manner. The cell viability was significantly(pinvestigate if a sub-lethal dose of cannabidiol when combined with cisplatin can induce a stronger cytotoxicity compared to the application of cis-platin alone
| Original language | English |
|---|---|
| Article number | POO97 |
| Pages (from-to) | 439 |
| Number of pages | 1 |
| Journal | British Journal of Pharmacology |
| Volume | 180 |
| Issue number | S1 |
| Early online date | 27 Jun 2023 |
| DOIs | |
| Publication status | Published - 1 Jul 2023 |
| Event | 19th World Congress of Basic & Clinical Pharmacology - Glasgow, United Kingdom Duration: 2 Jul 2023 → 7 Jul 2023 Conference number: 19 |
