TY - JOUR
T1 - The effect of 5-HT and electrical field stimulation on the contractility of the whole isolated urinary bladder of Suncus murinus
AU - Javid, Farideh A.
AU - Palea, Stefano
PY - 2014/1/15
Y1 - 2014/1/15
N2 - The present study used the whole isolated urinary bladder of Suncus murinus, to investigate the effect of exogenously added serotonin (5-HT) and electrical field stimulation (EFS) in the absence and presence of methysergide, a 5-HT1/2/7 receptor antagonist or the selective 5-HT7 receptor antagonist, SB269970. Further experiments investigated the involvement of potassium channel, cholinergic and purinergic systems in mediating the contractile response to EFS. Pre-treatment with methysergide reduced and increased the contractile responses to 5-HT and EFS, respectively. Pre-treatment with SB269970 increased the responses to 5-HT without modifying the EFS-induced contractions. EFS-induced contractions were not modified by pre-treatment with atropine (10 μM), α-β-methylene ATP or glibenclamide. EFS-induced contractions were attenuated by cromakalim (10 μM) or atropine (0.1 μM). In conclusion, the 5-HT2 receptors are likely to play a role in mediating the contractile response to 5-HT in detrusor muscle. Furthermore, EFS-induced contractions are mediated through cholinergic and an unknown neurotransmitter which is modulated by K(ATP) channels in the detrusor muscle of Suncus murinus.
AB - The present study used the whole isolated urinary bladder of Suncus murinus, to investigate the effect of exogenously added serotonin (5-HT) and electrical field stimulation (EFS) in the absence and presence of methysergide, a 5-HT1/2/7 receptor antagonist or the selective 5-HT7 receptor antagonist, SB269970. Further experiments investigated the involvement of potassium channel, cholinergic and purinergic systems in mediating the contractile response to EFS. Pre-treatment with methysergide reduced and increased the contractile responses to 5-HT and EFS, respectively. Pre-treatment with SB269970 increased the responses to 5-HT without modifying the EFS-induced contractions. EFS-induced contractions were not modified by pre-treatment with atropine (10 μM), α-β-methylene ATP or glibenclamide. EFS-induced contractions were attenuated by cromakalim (10 μM) or atropine (0.1 μM). In conclusion, the 5-HT2 receptors are likely to play a role in mediating the contractile response to 5-HT in detrusor muscle. Furthermore, EFS-induced contractions are mediated through cholinergic and an unknown neurotransmitter which is modulated by K(ATP) channels in the detrusor muscle of Suncus murinus.
KW - 5-Hydroxytryptamine
KW - Electrical field stimulation
KW - Purinergic P2X receptors
KW - Suncus murinus
KW - Urinary bladder
UR - http://www.scopus.com/inward/record.url?scp=84893778010&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2013.10.024
DO - 10.1016/j.ejphar.2013.10.024
M3 - Article
C2 - 24513135
AN - SCOPUS:84893778010
VL - 723
SP - 489
EP - 493
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -