The effect of 5-HT and electrical field stimulation on the contractility of the whole isolated urinary bladder of Suncus murinus

Farideh A. Javid, Stefano Palea

Research output: Contribution to journalArticle

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Abstract

The present study used the whole isolated urinary bladder of Suncus murinus, to investigate the effect of exogenously added serotonin (5-HT) and electrical field stimulation (EFS) in the absence and presence of methysergide, a 5-HT1/2/7 receptor antagonist or the selective 5-HT7 receptor antagonist, SB269970. Further experiments investigated the involvement of potassium channel, cholinergic and purinergic systems in mediating the contractile response to EFS. Pre-treatment with methysergide reduced and increased the contractile responses to 5-HT and EFS, respectively. Pre-treatment with SB269970 increased the responses to 5-HT without modifying the EFS-induced contractions. EFS-induced contractions were not modified by pre-treatment with atropine (10 μM), α-β-methylene ATP or glibenclamide. EFS-induced contractions were attenuated by cromakalim (10 μM) or atropine (0.1 μM). In conclusion, the 5-HT2 receptors are likely to play a role in mediating the contractile response to 5-HT in detrusor muscle. Furthermore, EFS-induced contractions are mediated through cholinergic and an unknown neurotransmitter which is modulated by K(ATP) channels in the detrusor muscle of Suncus murinus. 

Original languageEnglish
Pages (from-to)489-493
Number of pages5
JournalEuropean Journal of Pharmacology
Volume723
Issue number1
Early online date24 Oct 2013
DOIs
Publication statusPublished - 15 Jan 2014

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Electric Stimulation
Serotonin
Urinary Bladder
Methysergide
Atropine
Cholinergic Agents
Adenosine Triphosphate
Cromakalim
Muscles
Glyburide
Potassium Channels
Neurotransmitter Agents

Cite this

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abstract = "The present study used the whole isolated urinary bladder of Suncus murinus, to investigate the effect of exogenously added serotonin (5-HT) and electrical field stimulation (EFS) in the absence and presence of methysergide, a 5-HT1/2/7 receptor antagonist or the selective 5-HT7 receptor antagonist, SB269970. Further experiments investigated the involvement of potassium channel, cholinergic and purinergic systems in mediating the contractile response to EFS. Pre-treatment with methysergide reduced and increased the contractile responses to 5-HT and EFS, respectively. Pre-treatment with SB269970 increased the responses to 5-HT without modifying the EFS-induced contractions. EFS-induced contractions were not modified by pre-treatment with atropine (10 μM), α-β-methylene ATP or glibenclamide. EFS-induced contractions were attenuated by cromakalim (10 μM) or atropine (0.1 μM). In conclusion, the 5-HT2 receptors are likely to play a role in mediating the contractile response to 5-HT in detrusor muscle. Furthermore, EFS-induced contractions are mediated through cholinergic and an unknown neurotransmitter which is modulated by K(ATP) channels in the detrusor muscle of Suncus murinus. ",
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The effect of 5-HT and electrical field stimulation on the contractility of the whole isolated urinary bladder of Suncus murinus. / Javid, Farideh A.; Palea, Stefano.

In: European Journal of Pharmacology, Vol. 723, No. 1, 15.01.2014, p. 489-493.

Research output: Contribution to journalArticle

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