The effect of 5-HT and electrical field stimulation on the contractility of the whole isolated urinary bladder of Suncus murinus

The present study used the whole isolated urinary bladder of Suncus murinus, to investigate the effect of exogenously added serotonin (5-HT) and electrical field stimulation (EFS) in the absence and presence of methysergide, a 5-_HT1/2/7 receptor antagonist or the selective 5-HT₇ receptor antagonist, SB269970. Further experiments investigated the involvement of potassium channel, cholinergic and purinergic systems in mediating the contractile response to EFS. Pre-treatment with methysergide reduced and increased the contractile responses to 5-HT and EFS, respectively. Pre-treatment with SB269970 increased the responses to 5-HT without modifying the EFS-induced contractions. EFS-induced contractions were not modified by pre-treatment with atropine (10 μM), α-β-methylene ATP or glibenclamide. EFS-induced contractions were attenuated by cromakalim (10 μM) or atropine (0.1 μM). In conclusion, the 5-HT₂ receptors are likely to play a role in mediating the contractile response to 5-HT in detrusor muscle. Furthermore, EFS-induced contractions are mediated through cholinergic and an unknown neurotransmitter which is modulated by K(ATP) channels in the detrusor muscle of Suncus murinus.