TY - JOUR
T1 - The effects of blood conditioning films on the antimicrobial and retention properties of zirconium-nitride silver surfaces
AU - Slate, Anthony J.
AU - Wickens, David
AU - Wilson-Nieuwenhuis, Joels
AU - Dempsey-Hibbert, Nina
AU - West, Glen
AU - Kelly, Peter
AU - Verran, Joanna
AU - Banks, Craig E.
AU - Whitehead, Kathryn A.
N1 - Funding Information:
This work was funded by Manchester Metropolitan University .
Publisher Copyright:
© 2018 The Authors
PY - 2019/1/1
Y1 - 2019/1/1
N2 - External bone fixation devices provide support and rehabilitation for severely damaged/broken bones, however, this invasive procedure is prone to infection. Zirconium nitride/silver (Ti-ZrN/Ag) coatings were characterised for surface topography, chemical composition, physicochemistry and antimicrobial efficacy (against Staphylococcus aureus and Staphylococcus epidermidis), in the presence of a blood conditioning film. The conditioning film altered the width of the microtopography of the surfaces however, the depth of the features remained relatively constant. The conditioning film also altered the coatings from hydrophobic to hydrophilic/partially hydrophilic surfaces. Following the MATH assay, the presence of a conditioning film reduced affinity towards the hydrocarbons for both microorganisms. The addition of a blood conditioning film reduced the antimicrobial efficacy of the Ti-ZrN/Ag coatings but also reduced the number of retained bacteria. This study suggests that the presence of a pre-defined blood conditioning film may result in surfaces with anti-adhesive properties, potentially leading to a reduction in bacterial retention. This, combined with the antimicrobial efficacy of the coatings, could reduce the risk of infection on biomaterial surfaces.
AB - External bone fixation devices provide support and rehabilitation for severely damaged/broken bones, however, this invasive procedure is prone to infection. Zirconium nitride/silver (Ti-ZrN/Ag) coatings were characterised for surface topography, chemical composition, physicochemistry and antimicrobial efficacy (against Staphylococcus aureus and Staphylococcus epidermidis), in the presence of a blood conditioning film. The conditioning film altered the width of the microtopography of the surfaces however, the depth of the features remained relatively constant. The conditioning film also altered the coatings from hydrophobic to hydrophilic/partially hydrophilic surfaces. Following the MATH assay, the presence of a conditioning film reduced affinity towards the hydrocarbons for both microorganisms. The addition of a blood conditioning film reduced the antimicrobial efficacy of the Ti-ZrN/Ag coatings but also reduced the number of retained bacteria. This study suggests that the presence of a pre-defined blood conditioning film may result in surfaces with anti-adhesive properties, potentially leading to a reduction in bacterial retention. This, combined with the antimicrobial efficacy of the coatings, could reduce the risk of infection on biomaterial surfaces.
KW - Antimicrobial efficacy
KW - Blood conditioning films
KW - External bone fixation pins
KW - Physicochemistry
KW - Silver
KW - Zirconium nitride
UR - http://www.scopus.com/inward/record.url?scp=85054362905&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2018.09.060
DO - 10.1016/j.colsurfb.2018.09.060
M3 - Article
C2 - 30308455
AN - SCOPUS:85054362905
VL - 173
SP - 303
EP - 311
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -