The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations

Darren Greetham, Tithira T. Wimalasena, Kay Leung, Marcus E. Marvin, Yogeshwar Chandelia, Andrew J. Hart, Trevor G. Phister, Gregory A. Tucker, Edward J. Louis, Katherine A. Smart

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Saccharomyces cerevisiae is the micro-organism of choice for the conversion of monomeric sugars into bioethanol. Industrial bioethanol fermentations are intrinsically stressful environments for yeast and the adaptive protective response varies between strain backgrounds. With the aim of identifying quantitative trait loci (QTL's) that regulate phenotypic variation, linkage analysis on six F1 crosses from four highly divergent clean lineages of S. cerevisiae was performed. Segregants from each cross were assessed for tolerance to a range of stresses encountered during industrial bioethanol fermentations. Tolerance levels within populations of F1 segregants to stress conditions differed and displayed transgressive variation. Linkage analysis resulted in the identification of QTL's for tolerance to weak acid and osmotic stress. We tested candidate genes within loci identified by QTL using reciprocal hemizygosity analysis to ascertain their contribution to the observed phenotypic variation; this approach validated a gene (COX20) for weak acid stress and a gene (RCK2) for osmotic stress. Hemizygous transformants with a sensitive phenotype carried a COX20 allele from a weak acid sensitive parent with an alteration in its protein coding compared with other S. cerevisiae strains. RCK2 alleles reveal peptide differences between parental strains and the importance of these changes is currently being ascertained.
LanguageEnglish
Article numbere103233
Number of pages14
JournalPLoS One
Volume9
Issue number8
DOIs
Publication statusPublished - 12 Aug 2014
Externally publishedYes

Fingerprint

ethanol fermentation
Bioethanol
Quantitative Trait Loci
Yeast
Fermentation
Saccharomyces cerevisiae
quantitative trait loci
stress response
Osmotic Pressure
osmotic stress
phenotypic variation
Acids
linkage (genetics)
Genes
acids
Alleles
alleles
gene dosage
bioethanol
genes

Cite this

Greetham, Darren ; Wimalasena, Tithira T. ; Leung, Kay ; Marvin, Marcus E. ; Chandelia, Yogeshwar ; Hart, Andrew J. ; Phister, Trevor G. ; Tucker, Gregory A. ; Louis, Edward J. ; Smart, Katherine A. / The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations. In: PLoS One. 2014 ; Vol. 9, No. 8.
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Greetham, D, Wimalasena, TT, Leung, K, Marvin, ME, Chandelia, Y, Hart, AJ, Phister, TG, Tucker, GA, Louis, EJ & Smart, KA 2014, 'The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations', PLoS One, vol. 9, no. 8, e103233. https://doi.org/10.1371/journal.pone.0103233

The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations. / Greetham, Darren; Wimalasena, Tithira T.; Leung, Kay; Marvin, Marcus E.; Chandelia, Yogeshwar; Hart, Andrew J.; Phister, Trevor G.; Tucker, Gregory A.; Louis, Edward J.; Smart, Katherine A.

In: PLoS One, Vol. 9, No. 8, e103233, 12.08.2014.

Research output: Contribution to journalArticle

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