The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Lillian K. Fritz-Laylin, Simon E. Prochnik, Michael L. Ginger, Joel B. Dacks, Meredith L. Carpenter, Mark C. Field, Alan Kuo, Alex Paredez, Jarrod Chapman, Jonathan Pham, Shengqiang Shu, Rochak Neupane, Michael Cipriano, Joel Mancuso, Hank Tu, Asaf Salamov, Erika Lindquist, Harris Shapiro, Susan Lucas, Igor V. GrigorievW. Zacheus Cande, Chandler Fulton, Daniel S. Rokhsar, Scott C. Dawson

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.

Original languageEnglish
Pages (from-to)631-642
Number of pages12
JournalCell
Volume140
Issue number5
Early online date4 Mar 2010
DOIs
Publication statusPublished - 5 Mar 2010
Externally publishedYes

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    Fritz-Laylin, L. K., Prochnik, S. E., Ginger, M. L., Dacks, J. B., Carpenter, M. L., Field, M. C., Kuo, A., Paredez, A., Chapman, J., Pham, J., Shu, S., Neupane, R., Cipriano, M., Mancuso, J., Tu, H., Salamov, A., Lindquist, E., Shapiro, H., Lucas, S., ... Dawson, S. C. (2010). The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility. Cell, 140(5), 631-642. https://doi.org/10.1016/j.cell.2010.01.032