The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Lillian K. Fritz-Laylin; Simon E. Prochnik; Michael L. Ginger; Joel B. Dacks; Meredith L. Carpenter; Mark C. Field; Alan Kuo; Alex Paredez; Jarrod Chapman; Jonathan Pham; Shengqiang Shu; Rochak Neupane; Michael Cipriano; Joel Mancuso; Hank Tu; Asaf Salamov; Erika Lindquist; Harris Shapiro; Susan Lucas; Igor V. Grigoriev; W. Zacheus Cande; Chandler Fulton; Daniel S. Rokhsar; Scott C. Dawson

doi:10.1016/j.cell.2010.01.032

The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Lillian K. Fritz-Laylin, Simon E. Prochnik, Michael L. Ginger, Joel B. Dacks, Meredith L. Carpenter, Mark C. Field, Alan Kuo, Alex Paredez, Jarrod Chapman, Jonathan Pham, Shengqiang Shu, Rochak Neupane, Michael Cipriano, Joel Mancuso, Hank Tu, Asaf Salamov, Erika Lindquist, Harris Shapiro, Susan Lucas, Igor V. GrigorievW. Zacheus Cande, Chandler Fulton, Daniel S. Rokhsar, Scott C. Dawson

Research output: Contribution to journal › Article › peer-review

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Abstract

Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.

Original language	English
Pages (from-to)	631-642
Number of pages	12
Journal	Cell
Volume	140
Issue number	5
Early online date	4 Mar 2010
DOIs	https://doi.org/10.1016/j.cell.2010.01.032
Publication status	Published - 5 Mar 2010
Externally published	Yes

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Fritz-Laylin, L. K., Prochnik, S. E., Ginger, M. L., Dacks, J. B., Carpenter, M. L., Field, M. C., Kuo, A., Paredez, A., Chapman, J., Pham, J., Shu, S., Neupane, R., Cipriano, M., Mancuso, J., Tu, H., Salamov, A., Lindquist, E., Shapiro, H., Lucas, S., ... Dawson, S. C. (2010). The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility. Cell, 140(5), 631-642. https://doi.org/10.1016/j.cell.2010.01.032

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title = "The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility",

abstract = "Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.",

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author = "Fritz-Laylin, {Lillian K.} and Prochnik, {Simon E.} and Ginger, {Michael L.} and Dacks, {Joel B.} and Carpenter, {Meredith L.} and Field, {Mark C.} and Alan Kuo and Alex Paredez and Jarrod Chapman and Jonathan Pham and Shengqiang Shu and Rochak Neupane and Michael Cipriano and Joel Mancuso and Hank Tu and Asaf Salamov and Erika Lindquist and Harris Shapiro and Susan Lucas and Grigoriev, {Igor V.} and Cande, {W. Zacheus} and Chandler Fulton and Rokhsar, {Daniel S.} and Dawson, {Scott C.}",

year = "2010",

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doi = "10.1016/j.cell.2010.01.032",

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Fritz-Laylin, LK, Prochnik, SE, Ginger, ML, Dacks, JB, Carpenter, ML, Field, MC, Kuo, A, Paredez, A, Chapman, J, Pham, J, Shu, S, Neupane, R, Cipriano, M, Mancuso, J, Tu, H, Salamov, A, Lindquist, E, Shapiro, H, Lucas, S, Grigoriev, IV, Cande, WZ, Fulton, C, Rokhsar, DS & Dawson, SC 2010, 'The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility', Cell, vol. 140, no. 5, pp. 631-642. https://doi.org/10.1016/j.cell.2010.01.032

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AU - Fritz-Laylin, Lillian K.

AU - Prochnik, Simon E.

AU - Ginger, Michael L.

AU - Dacks, Joel B.

AU - Carpenter, Meredith L.

AU - Field, Mark C.

AU - Kuo, Alan

AU - Paredez, Alex

AU - Chapman, Jarrod

AU - Pham, Jonathan

AU - Shu, Shengqiang

AU - Neupane, Rochak

AU - Cipriano, Michael

AU - Mancuso, Joel

AU - Tu, Hank

AU - Salamov, Asaf

AU - Lindquist, Erika

AU - Shapiro, Harris

AU - Lucas, Susan

AU - Grigoriev, Igor V.

AU - Cande, W. Zacheus

AU - Fulton, Chandler

AU - Rokhsar, Daniel S.

AU - Dawson, Scott C.

PY - 2010/3/5

Y1 - 2010/3/5

N2 - Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.

AB - Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.

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DO - 10.1016/j.cell.2010.01.032

M3 - Article

C2 - 20211133

AN - SCOPUS:77649290275

VL - 140

SP - 631

EP - 642

JO - Cell

JF - Cell

SN - 0092-8674

IS - 5

ER -

The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

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