The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Lillian K. Fritz-Laylin, Simon E. Prochnik, Michael L. Ginger, Joel B. Dacks, Meredith L. Carpenter, Mark C. Field, Alan Kuo, Alex Paredez, Jarrod Chapman, Jonathan Pham, Shengqiang Shu, Rochak Neupane, Michael Cipriano, Joel Mancuso, Hank Tu, Asaf Salamov, Erika Lindquist, Harris Shapiro, Susan Lucas, Igor V. Grigoriev & 4 others W. Zacheus Cande, Chandler Fulton, Daniel S. Rokhsar, Scott C. Dawson

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.

LanguageEnglish
Pages631-642
Number of pages12
JournalCell
Volume140
Issue number5
Early online date4 Mar 2010
DOIs
Publication statusPublished - 5 Mar 2010
Externally publishedYes

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Naegleria
Genes
Genome
Eukaryota
Anaerobiosis
Metabolism
Organelles
Cell Biology
Molecular Biology
Hydrogen
Respiration
Cytology
Molecular biology
Patents and inventions
Hydrogen production
Proteins

Cite this

Fritz-Laylin, L. K., Prochnik, S. E., Ginger, M. L., Dacks, J. B., Carpenter, M. L., Field, M. C., ... Dawson, S. C. (2010). The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility. Cell, 140(5), 631-642. https://doi.org/10.1016/j.cell.2010.01.032
Fritz-Laylin, Lillian K. ; Prochnik, Simon E. ; Ginger, Michael L. ; Dacks, Joel B. ; Carpenter, Meredith L. ; Field, Mark C. ; Kuo, Alan ; Paredez, Alex ; Chapman, Jarrod ; Pham, Jonathan ; Shu, Shengqiang ; Neupane, Rochak ; Cipriano, Michael ; Mancuso, Joel ; Tu, Hank ; Salamov, Asaf ; Lindquist, Erika ; Shapiro, Harris ; Lucas, Susan ; Grigoriev, Igor V. ; Cande, W. Zacheus ; Fulton, Chandler ; Rokhsar, Daniel S. ; Dawson, Scott C. / The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility. In: Cell. 2010 ; Vol. 140, No. 5. pp. 631-642.
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Fritz-Laylin, LK, Prochnik, SE, Ginger, ML, Dacks, JB, Carpenter, ML, Field, MC, Kuo, A, Paredez, A, Chapman, J, Pham, J, Shu, S, Neupane, R, Cipriano, M, Mancuso, J, Tu, H, Salamov, A, Lindquist, E, Shapiro, H, Lucas, S, Grigoriev, IV, Cande, WZ, Fulton, C, Rokhsar, DS & Dawson, SC 2010, 'The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility', Cell, vol. 140, no. 5, pp. 631-642. https://doi.org/10.1016/j.cell.2010.01.032

The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility. / Fritz-Laylin, Lillian K.; Prochnik, Simon E.; Ginger, Michael L.; Dacks, Joel B.; Carpenter, Meredith L.; Field, Mark C.; Kuo, Alan; Paredez, Alex; Chapman, Jarrod; Pham, Jonathan; Shu, Shengqiang; Neupane, Rochak; Cipriano, Michael; Mancuso, Joel; Tu, Hank; Salamov, Asaf; Lindquist, Erika; Shapiro, Harris; Lucas, Susan; Grigoriev, Igor V.; Cande, W. Zacheus; Fulton, Chandler; Rokhsar, Daniel S.; Dawson, Scott C.

In: Cell, Vol. 140, No. 5, 05.03.2010, p. 631-642.

Research output: Contribution to journalArticle

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AU - Carpenter, Meredith L.

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AU - Mancuso, Joel

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AU - Grigoriev, Igor V.

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AU - Rokhsar, Daniel S.

AU - Dawson, Scott C.

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Fritz-Laylin LK, Prochnik SE, Ginger ML, Dacks JB, Carpenter ML, Field MC et al. The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility. Cell. 2010 Mar 5;140(5):631-642. https://doi.org/10.1016/j.cell.2010.01.032