The Holozoan capsaspora owczarzaki possesses a diverse complement of active transposable element families

Martin Carr, Hiroshi Suga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Capsaspora owczarzaki, a protistan symbiont of the pulmonate snail Biomphalaria glabrata, is the centre of much interest in evolutionary biology due to its close relationship toMetazoa. Thewhole genome sequence of this protist has revealednewinsights into the ancestral genome composition of Metazoa, in particular with regard to gene families involved in the evolution ofmulticellularity. The draft genome revealed the presence of 23 families of transposable element,made up from DNA transposon as well as long terminal repeat (LTR) and non-LTR retrotransposon families. The phylogenetic analyses presented here show that all of the transposable elements identified in the C. owczarzaki genome have orthologous families inMetazoa, indicating that the ancestralmetazoan also had a rich diversity of elements. Molecular evolutionary analyses also showthat the majority of families has recently been active within the Capsaspora genome.One familynowappears to be inactive and a further five families showno evidence of current transposition. Most individual element copies are evolutionarily young; however, a small proportion of inserts appear to have persisted for longer in thegenome.Thefamiliespresent in thegenomeshowcontrastingpopulationhistories and appear to be in different stages of their life cycles. Transcriptome data have been analyzed frommultiple stages in the C. owczarzaki life cycle. Expression levels vary greatly both between families and between different stages of the life cycle, suggesting an unexpectedly complex level of transposable element regulation in a single celled organism.

Original languageEnglish
Pages (from-to)949-963
Number of pages15
JournalGenome Biology and Evolution
Volume6
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

DNA Transposable Elements
transposons
complement
Genome
Life Cycle Stages
genome
terminal repeat sequences
Terminal Repeat Sequences
life cycle
developmental stages
Biomphalaria
Biomphalaria glabrata
Retroelements
Animalia
transposition (genetics)
Snails
retrotransposons
Transcriptome
transcriptome
symbionts

Cite this

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title = "The Holozoan capsaspora owczarzaki possesses a diverse complement of active transposable element families",
abstract = "Capsaspora owczarzaki, a protistan symbiont of the pulmonate snail Biomphalaria glabrata, is the centre of much interest in evolutionary biology due to its close relationship toMetazoa. Thewhole genome sequence of this protist has revealednewinsights into the ancestral genome composition of Metazoa, in particular with regard to gene families involved in the evolution ofmulticellularity. The draft genome revealed the presence of 23 families of transposable element,made up from DNA transposon as well as long terminal repeat (LTR) and non-LTR retrotransposon families. The phylogenetic analyses presented here show that all of the transposable elements identified in the C. owczarzaki genome have orthologous families inMetazoa, indicating that the ancestralmetazoan also had a rich diversity of elements. Molecular evolutionary analyses also showthat the majority of families has recently been active within the Capsaspora genome.One familynowappears to be inactive and a further five families showno evidence of current transposition. Most individual element copies are evolutionarily young; however, a small proportion of inserts appear to have persisted for longer in thegenome.Thefamiliespresent in thegenomeshowcontrastingpopulationhistories and appear to be in different stages of their life cycles. Transcriptome data have been analyzed frommultiple stages in the C. owczarzaki life cycle. Expression levels vary greatly both between families and between different stages of the life cycle, suggesting an unexpectedly complex level of transposable element regulation in a single celled organism.",
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The Holozoan capsaspora owczarzaki possesses a diverse complement of active transposable element families. / Carr, Martin; Suga, Hiroshi.

In: Genome Biology and Evolution, Vol. 6, No. 4, 2014, p. 949-963.

Research output: Contribution to journalArticle

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