The isolation of peste des petits ruminants virus from northern India

Y P Nanda; A Chatterjee; A K Purohit; A Diallo; K Innui; R N Sharma; G Libeau; J A Thevasagayam; A Brüning; R P Kitching; J Anderson; T Barrett; W P Taylor

doi:10.1016/0378-1135(96)00025-9

The isolation of peste des petits ruminants virus from northern India

Y P Nanda, A Chatterjee, A K Purohit, A Diallo, K Innui, R N Sharma, G Libeau, J A Thevasagayam, A Brüning, R P Kitching, J Anderson, T Barrett, W P Taylor

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Abstract

The aetiological agent responsible for an epizootic of a rinderpest-like disease afflicting sheep and goats in three states of northern India was confirmed as peste des petits ruminants virus. To differentiate the virus from rinderpest a number of diagnostic tests were used, including immunocapture ELISA, specific oligonucleotide primers in a reverse transcriptase polymerase chain reaction, immunofluorescence with virus specific monoclonal antibodies and virus isolation. The virulence profile of one isolate in cattle sheep and goats was established. Infected animals developed specific antibody responses and excreted specific antigen in their lachrymal secretions.

Original language	English
Pages (from-to)	207-216
Number of pages	10
Journal	Veterinary Microbiology
Volume	51
Issue number	3-4
DOIs	https://doi.org/10.1016/0378-1135(96)00025-9
Publication status	Published - Aug 1996
Externally published	Yes

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title = "The isolation of peste des petits ruminants virus from northern India",

abstract = "The aetiological agent responsible for an epizootic of a rinderpest-like disease afflicting sheep and goats in three states of northern India was confirmed as peste des petits ruminants virus. To differentiate the virus from rinderpest a number of diagnostic tests were used, including immunocapture ELISA, specific oligonucleotide primers in a reverse transcriptase polymerase chain reaction, immunofluorescence with virus specific monoclonal antibodies and virus isolation. The virulence profile of one isolate in cattle sheep and goats was established. Infected animals developed specific antibody responses and excreted specific antigen in their lachrymal secretions.",

keywords = "Animals, Antibodies, Monoclonal, Antibodies, Viral/biosynthesis, Antibody Formation, Cattle, Cattle Diseases, DNA Primers, Enzyme-Linked Immunosorbent Assay/methods, Fluorescent Antibody Technique, Goat Diseases, Goats, India, Molecular Sequence Data, Morbillivirus Infections/diagnosis, Peste-des-petits-ruminants virus/isolation & purification, Polymerase Chain Reaction/methods, Sheep, Sheep Diseases",

author = "Nanda, {Y P} and A Chatterjee and Purohit, {A K} and A Diallo and K Innui and Sharma, {R N} and G Libeau and Thevasagayam, {J A} and A Br{\"u}ning and Kitching, {R P} and J Anderson and T Barrett and Taylor, {W P}",

year = "1996",

month = aug,

doi = "10.1016/0378-1135(96)00025-9",

language = "English",

volume = "51",

pages = "207--216",

journal = "Veterinary Microbiology",

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TY - JOUR

T1 - The isolation of peste des petits ruminants virus from northern India

AU - Nanda, Y P

AU - Chatterjee, A

AU - Purohit, A K

AU - Diallo, A

AU - Innui, K

AU - Sharma, R N

AU - Libeau, G

AU - Thevasagayam, J A

AU - Brüning, A

AU - Kitching, R P

AU - Anderson, J

AU - Barrett, T

AU - Taylor, W P

PY - 1996/8

Y1 - 1996/8

N2 - The aetiological agent responsible for an epizootic of a rinderpest-like disease afflicting sheep and goats in three states of northern India was confirmed as peste des petits ruminants virus. To differentiate the virus from rinderpest a number of diagnostic tests were used, including immunocapture ELISA, specific oligonucleotide primers in a reverse transcriptase polymerase chain reaction, immunofluorescence with virus specific monoclonal antibodies and virus isolation. The virulence profile of one isolate in cattle sheep and goats was established. Infected animals developed specific antibody responses and excreted specific antigen in their lachrymal secretions.

AB - The aetiological agent responsible for an epizootic of a rinderpest-like disease afflicting sheep and goats in three states of northern India was confirmed as peste des petits ruminants virus. To differentiate the virus from rinderpest a number of diagnostic tests were used, including immunocapture ELISA, specific oligonucleotide primers in a reverse transcriptase polymerase chain reaction, immunofluorescence with virus specific monoclonal antibodies and virus isolation. The virulence profile of one isolate in cattle sheep and goats was established. Infected animals developed specific antibody responses and excreted specific antigen in their lachrymal secretions.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antibodies, Viral/biosynthesis

KW - Antibody Formation

KW - Cattle

KW - Cattle Diseases

KW - DNA Primers

KW - Enzyme-Linked Immunosorbent Assay/methods

KW - Fluorescent Antibody Technique

KW - Goat Diseases

KW - Goats

KW - India

KW - Molecular Sequence Data

KW - Morbillivirus Infections/diagnosis

KW - Peste-des-petits-ruminants virus/isolation & purification

KW - Polymerase Chain Reaction/methods

KW - Sheep

KW - Sheep Diseases

U2 - 10.1016/0378-1135(96)00025-9

DO - 10.1016/0378-1135(96)00025-9

M3 - Article

C2 - 8870184

VL - 51

SP - 207

EP - 216

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -

The isolation of peste des petits ruminants virus from northern India

Abstract

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