The physicochemical characterisation of pepsin degraded pig gastric mucin

Atiga Abodinar, Kristoffer Tømmeraas, Elena Ronander, Alan M. Smith, Gordon A. Morris

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mucins are the main macromolecular components of the mucus secretions that cover the oral cavity, gastrointestinal and urogenital tracts of animals. The properties of the mucus secretions are therefore directly correlated with the physicochemical properties of mucin glycoproteins. In this study, mucins were obtained from pig gastric mucous after digestion with pepsin at 37 °C for 4 h, these mucins were characterised in terms of compositional and hydrodynamic properties.Compositional analysis showed that this mucin contains protein (15%), carbohydrates (55%) of which the constituents are: fucose (4%), galactose (9%), glucosamine (55%), glucosamine (33%) and sialic acid (2%). The latter component gives the mucin polymer a pH-dependant negative charge, with a ζ-potential of -3 mV at pH 1.2 up to -11 mV at pH 7.4. The weight average molar mass was ~1 × 106 g/mol and intrinsic viscosity was ~0.42 dL/g although there was a small pH dependency due to the polyelectrolyte behavior of the polymer. The measurements of viscosity versus shear rate showed shear thinning behavior and the critical overlap concentration was determined to be 10-11% w/v indicating a compact structure. Knowledge of these properties is fundamental to the understanding interactions of mucins, with for example, novel drug delivery systems.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalInternational Journal of Biological Macromolecules
Volume87
Early online date2 Mar 2016
DOIs
Publication statusPublished - 1 Jun 2016

Fingerprint

Gastric Mucins
Glucosamine
Pepsin A
Mucins
Swine
Viscosity
Glycoproteins
Shear thinning
Molar mass
Polymers
Carbohydrates
Polyelectrolytes
Shear deformation
Animals
Hydrodynamics
Mucus
Proteins
Acids
Mucin-4
Fucose

Cite this

@article{7e8f2490601b42e89105ee8a73e301d6,
title = "The physicochemical characterisation of pepsin degraded pig gastric mucin",
abstract = "Mucins are the main macromolecular components of the mucus secretions that cover the oral cavity, gastrointestinal and urogenital tracts of animals. The properties of the mucus secretions are therefore directly correlated with the physicochemical properties of mucin glycoproteins. In this study, mucins were obtained from pig gastric mucous after digestion with pepsin at 37 °C for 4 h, these mucins were characterised in terms of compositional and hydrodynamic properties.Compositional analysis showed that this mucin contains protein (15{\%}), carbohydrates (55{\%}) of which the constituents are: fucose (4{\%}), galactose (9{\%}), glucosamine (55{\%}), glucosamine (33{\%}) and sialic acid (2{\%}). The latter component gives the mucin polymer a pH-dependant negative charge, with a ζ-potential of -3 mV at pH 1.2 up to -11 mV at pH 7.4. The weight average molar mass was ~1 × 106 g/mol and intrinsic viscosity was ~0.42 dL/g although there was a small pH dependency due to the polyelectrolyte behavior of the polymer. The measurements of viscosity versus shear rate showed shear thinning behavior and the critical overlap concentration was determined to be 10-11{\%} w/v indicating a compact structure. Knowledge of these properties is fundamental to the understanding interactions of mucins, with for example, novel drug delivery systems.",
keywords = "Compact conformation, Pepsin degraded mucin, Physicochemical properties",
author = "Atiga Abodinar and Kristoffer T{\o}mmeraas and Elena Ronander and Smith, {Alan M.} and Morris, {Gordon A.}",
year = "2016",
month = "6",
day = "1",
doi = "10.1016/j.ijbiomac.2016.02.062",
language = "English",
volume = "87",
pages = "281--286",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",

}

The physicochemical characterisation of pepsin degraded pig gastric mucin. / Abodinar, Atiga; Tømmeraas, Kristoffer; Ronander, Elena; Smith, Alan M.; Morris, Gordon A.

In: International Journal of Biological Macromolecules, Vol. 87, 01.06.2016, p. 281-286.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The physicochemical characterisation of pepsin degraded pig gastric mucin

AU - Abodinar, Atiga

AU - Tømmeraas, Kristoffer

AU - Ronander, Elena

AU - Smith, Alan M.

AU - Morris, Gordon A.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Mucins are the main macromolecular components of the mucus secretions that cover the oral cavity, gastrointestinal and urogenital tracts of animals. The properties of the mucus secretions are therefore directly correlated with the physicochemical properties of mucin glycoproteins. In this study, mucins were obtained from pig gastric mucous after digestion with pepsin at 37 °C for 4 h, these mucins were characterised in terms of compositional and hydrodynamic properties.Compositional analysis showed that this mucin contains protein (15%), carbohydrates (55%) of which the constituents are: fucose (4%), galactose (9%), glucosamine (55%), glucosamine (33%) and sialic acid (2%). The latter component gives the mucin polymer a pH-dependant negative charge, with a ζ-potential of -3 mV at pH 1.2 up to -11 mV at pH 7.4. The weight average molar mass was ~1 × 106 g/mol and intrinsic viscosity was ~0.42 dL/g although there was a small pH dependency due to the polyelectrolyte behavior of the polymer. The measurements of viscosity versus shear rate showed shear thinning behavior and the critical overlap concentration was determined to be 10-11% w/v indicating a compact structure. Knowledge of these properties is fundamental to the understanding interactions of mucins, with for example, novel drug delivery systems.

AB - Mucins are the main macromolecular components of the mucus secretions that cover the oral cavity, gastrointestinal and urogenital tracts of animals. The properties of the mucus secretions are therefore directly correlated with the physicochemical properties of mucin glycoproteins. In this study, mucins were obtained from pig gastric mucous after digestion with pepsin at 37 °C for 4 h, these mucins were characterised in terms of compositional and hydrodynamic properties.Compositional analysis showed that this mucin contains protein (15%), carbohydrates (55%) of which the constituents are: fucose (4%), galactose (9%), glucosamine (55%), glucosamine (33%) and sialic acid (2%). The latter component gives the mucin polymer a pH-dependant negative charge, with a ζ-potential of -3 mV at pH 1.2 up to -11 mV at pH 7.4. The weight average molar mass was ~1 × 106 g/mol and intrinsic viscosity was ~0.42 dL/g although there was a small pH dependency due to the polyelectrolyte behavior of the polymer. The measurements of viscosity versus shear rate showed shear thinning behavior and the critical overlap concentration was determined to be 10-11% w/v indicating a compact structure. Knowledge of these properties is fundamental to the understanding interactions of mucins, with for example, novel drug delivery systems.

KW - Compact conformation

KW - Pepsin degraded mucin

KW - Physicochemical properties

UR - http://www.scopus.com/inward/record.url?scp=84959363171&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2016.02.062

DO - 10.1016/j.ijbiomac.2016.02.062

M3 - Article

VL - 87

SP - 281

EP - 286

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -