The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2

Daniel A Patten, Shaun Leivers, Marcus J Chadha, Mohammed Maqsood, Paul N Humphreys, Andrew P Laws, Andrew Collett

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Abstract

The Lactic acid bacteria (LAB) Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski both secrete exopolysaccharides (EPSs) into their surrounding environments during growth. A number of EPSs have previously been shown to exhibit immunomodulatory activity with professional immune cells, such as macrophages, but only limited studies have been reported of their interaction with intestinal epithelial cells. An investigation of the immunomodulatory potential of pure EPSs, isolated from cultures of Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski, with the HT29-19A intestinal epithelial cell line are reported here. For the first time the structure of the EPS from Lactobacillus helveticus sp. Rosyjski which is a hetropolysaccharide with a branched pentasaccharide repeat unit containing d-glucose, d-galactose and N-acetyl-d-mannosamine is described. In response to exposure to lactobacilli EPSs HT29-19A cells produce significantly increased levels of the proinflammatory cytokine IL-8. Additionally, the EPSs differentially modulate the mRNA expression of Toll-like receptors. Finally, the pre-treatment of HT29-19A cells with the EPSs sensitises the cells to subsequent challenge with bacterial antigens. The results reported here suggest that EPSs could potentially play a role in intestinal homeostasis via a specific interaction with intestinal epithelial cells.

LanguageEnglish
Pages119-27
Number of pages9
JournalCarbohydrate Research
Volume384
DOIs
Publication statusPublished - 30 Jan 2014

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Lactobacillus helveticus
Lactobacillus acidophilus
HT29 Cells
Epithelial Cells
Bacterial Antigens
Macrophages
Toll-Like Receptors
Lactobacillus
Interleukin-8
Galactose
Lactic Acid
Bacteria
Homeostasis
Cytokines
Glucose
Cell Line
Messenger RNA
Growth

Cite this

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title = "The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2",
abstract = "The Lactic acid bacteria (LAB) Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski both secrete exopolysaccharides (EPSs) into their surrounding environments during growth. A number of EPSs have previously been shown to exhibit immunomodulatory activity with professional immune cells, such as macrophages, but only limited studies have been reported of their interaction with intestinal epithelial cells. An investigation of the immunomodulatory potential of pure EPSs, isolated from cultures of Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski, with the HT29-19A intestinal epithelial cell line are reported here. For the first time the structure of the EPS from Lactobacillus helveticus sp. Rosyjski which is a hetropolysaccharide with a branched pentasaccharide repeat unit containing d-glucose, d-galactose and N-acetyl-d-mannosamine is described. In response to exposure to lactobacilli EPSs HT29-19A cells produce significantly increased levels of the proinflammatory cytokine IL-8. Additionally, the EPSs differentially modulate the mRNA expression of Toll-like receptors. Finally, the pre-treatment of HT29-19A cells with the EPSs sensitises the cells to subsequent challenge with bacterial antigens. The results reported here suggest that EPSs could potentially play a role in intestinal homeostasis via a specific interaction with intestinal epithelial cells.",
keywords = "Carbohydrate Conformation, Epithelial Cells, HT29 Cells, Humans, Immunomodulation, Intestines, Lactobacillus acidophilus, Lactobacillus helveticus, Polysaccharides, Bacterial, Journal Article, Research Support, Non-U.S. Gov't",
author = "Patten, {Daniel A} and Shaun Leivers and Chadha, {Marcus J} and Mohammed Maqsood and Humphreys, {Paul N} and Laws, {Andrew P} and Andrew Collett",
note = "Copyright {\circledC} 2013 Elsevier Ltd. All rights reserved.",
year = "2014",
month = "1",
day = "30",
doi = "10.1016/j.carres.2013.12.008",
language = "English",
volume = "384",
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T1 - The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2

AU - Patten, Daniel A

AU - Leivers, Shaun

AU - Chadha, Marcus J

AU - Maqsood, Mohammed

AU - Humphreys, Paul N

AU - Laws, Andrew P

AU - Collett, Andrew

N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.

PY - 2014/1/30

Y1 - 2014/1/30

N2 - The Lactic acid bacteria (LAB) Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski both secrete exopolysaccharides (EPSs) into their surrounding environments during growth. A number of EPSs have previously been shown to exhibit immunomodulatory activity with professional immune cells, such as macrophages, but only limited studies have been reported of their interaction with intestinal epithelial cells. An investigation of the immunomodulatory potential of pure EPSs, isolated from cultures of Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski, with the HT29-19A intestinal epithelial cell line are reported here. For the first time the structure of the EPS from Lactobacillus helveticus sp. Rosyjski which is a hetropolysaccharide with a branched pentasaccharide repeat unit containing d-glucose, d-galactose and N-acetyl-d-mannosamine is described. In response to exposure to lactobacilli EPSs HT29-19A cells produce significantly increased levels of the proinflammatory cytokine IL-8. Additionally, the EPSs differentially modulate the mRNA expression of Toll-like receptors. Finally, the pre-treatment of HT29-19A cells with the EPSs sensitises the cells to subsequent challenge with bacterial antigens. The results reported here suggest that EPSs could potentially play a role in intestinal homeostasis via a specific interaction with intestinal epithelial cells.

AB - The Lactic acid bacteria (LAB) Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski both secrete exopolysaccharides (EPSs) into their surrounding environments during growth. A number of EPSs have previously been shown to exhibit immunomodulatory activity with professional immune cells, such as macrophages, but only limited studies have been reported of their interaction with intestinal epithelial cells. An investigation of the immunomodulatory potential of pure EPSs, isolated from cultures of Lactobacillus acidophilus sp. 5e2 and Lactobacillus helveticus sp. Rosyjski, with the HT29-19A intestinal epithelial cell line are reported here. For the first time the structure of the EPS from Lactobacillus helveticus sp. Rosyjski which is a hetropolysaccharide with a branched pentasaccharide repeat unit containing d-glucose, d-galactose and N-acetyl-d-mannosamine is described. In response to exposure to lactobacilli EPSs HT29-19A cells produce significantly increased levels of the proinflammatory cytokine IL-8. Additionally, the EPSs differentially modulate the mRNA expression of Toll-like receptors. Finally, the pre-treatment of HT29-19A cells with the EPSs sensitises the cells to subsequent challenge with bacterial antigens. The results reported here suggest that EPSs could potentially play a role in intestinal homeostasis via a specific interaction with intestinal epithelial cells.

KW - Carbohydrate Conformation

KW - Epithelial Cells

KW - HT29 Cells

KW - Humans

KW - Immunomodulation

KW - Intestines

KW - Lactobacillus acidophilus

KW - Lactobacillus helveticus

KW - Polysaccharides, Bacterial

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.carres.2013.12.008

DO - 10.1016/j.carres.2013.12.008

M3 - Article

VL - 384

SP - 119

EP - 127

JO - Carbohydrate Research

T2 - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

ER -