Tolerance of pentose utilising yeast to hydrogen peroxide-induced oxidative stress

Jennifer Spencer, Trevor G. Phister, Katherine A. Smart, Darren Greetham

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Bioethanol fermentations follow traditional beverage fermentations where the yeast is exposed to adverse conditions such as oxidative stress. Lignocellulosic bioethanol fermentations involve the conversion of pentose and hexose sugars into ethanol. Environmental stress conditions such as osmotic stress and ethanol stress may affect the fermentation performance; however, oxidative stress as a consequence of metabolic output can also occur. However, the effect of oxidative stress on yeast with pentose utilising capabilities has yet to be investigated. Results: Assaying for the effect of hydrogen peroxide-induced oxidative stress on Candida, Pichia and Scheffersomyces spp. has demonstrated that these yeast tolerate hydrogen peroxide-induced oxidative stress in a manner consistent with that demonstrated by Saccharomyces cerevisiae. Pichia guillermondii appears to be more tolerant to hydrogen peroxide-induced oxidative stress when compared to Candida shehatae, Candida succiphila or Scheffersomyces stipitis. Conclusions: Sensitivity to hydrogen peroxide-induced oxidative stress increased in the presence of minimal media; however, addition of amino acids and nucleobases was observed to increase tolerance. In particular adenine increased tolerance and methionine reduced tolerance to hydrogen peroxide-induced oxidative stress.

Original languageEnglish
Article number151
Number of pages12
JournalBMC Research Notes
Volume7
Issue number1
DOIs
Publication statusPublished - 17 Mar 2014
Externally publishedYes

Fingerprint

Pentoses
Oxidative stress
Yeast
Hydrogen Peroxide
Oxidative Stress
Yeasts
Fermentation
Candida
Bioethanol
Pichia
Ethanol
Hexoses
Beverages
Osmotic Pressure
Adenine
Sugars
Methionine
Saccharomyces cerevisiae
Amino Acids

Cite this

Spencer, Jennifer ; Phister, Trevor G. ; Smart, Katherine A. ; Greetham, Darren. / Tolerance of pentose utilising yeast to hydrogen peroxide-induced oxidative stress. In: BMC Research Notes. 2014 ; Vol. 7, No. 1.
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Tolerance of pentose utilising yeast to hydrogen peroxide-induced oxidative stress. / Spencer, Jennifer; Phister, Trevor G.; Smart, Katherine A.; Greetham, Darren.

In: BMC Research Notes, Vol. 7, No. 1, 151, 17.03.2014.

Research output: Contribution to journalArticle

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