Trypanosomatids: Methods and Protocols

Paul A M Michels; Michael Ginger; Dan Zilberstein

doi:10.1007/978-1-0716-0294-2

Trypanosomatids: Methods and Protocols

Paul A M Michels (Editor), Michael Ginger (Editor), Dan Zilberstein (Editor)

Research output: Book/Report › Book › peer-review

Abstract

This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases.

Original language	English
Publisher	Humana Press
Number of pages	823
Volume	2116
ISBN (Electronic)	9781071602942
ISBN (Print)	9781071602935
DOIs	https://doi.org/10.1007/978-1-0716-0294-2
Publication status	Published - 28 Mar 2020

Publication series

Name	Methods in Molecular Biology
Publisher	Humana
Volume	2116
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-0716-0294-2Licence: Unspecified

Cite this

@book{039b4c9bc28147c2977f7a05d588ac8d,

title = "Trypanosomatids: Methods and Protocols",

abstract = "This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases. ",

keywords = "parasite infections, drug discovery, Leishmania, protists, animal models, T. brucei, CRISPR/Cas9, reverse genetics",

editor = "Michels, {Paul A M} and Michael Ginger and Dan Zilberstein",

year = "2020",

month = mar,

day = "28",

doi = "10.1007/978-1-0716-0294-2",

language = "English",

isbn = "9781071602935",

volume = "2116",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

address = "United States",

}

TY - BOOK

T1 - Trypanosomatids

T2 - Methods and Protocols

A2 - Michels, Paul A M

A2 - Ginger, Michael

A2 - Zilberstein, Dan

PY - 2020/3/28

Y1 - 2020/3/28

N2 - This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases.

AB - This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases.

KW - parasite infections

KW - drug discovery

KW - Leishmania

KW - protists

KW - animal models

KW - T. brucei

KW - CRISPR/Cas9

KW - reverse genetics

U2 - 10.1007/978-1-0716-0294-2

DO - 10.1007/978-1-0716-0294-2

M3 - Book

SN - 9781071602935

VL - 2116

T3 - Methods in Molecular Biology

BT - Trypanosomatids

PB - Humana Press

ER -

Trypanosomatids: Methods and Protocols

Abstract

Publication series

UN SDGs

Access to Document

Fingerprint

Cite this