Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation

Jacqueline A. Davidge, Emma Chambers, Harriet A. Dickinson, Katie Towers, Michael L. Ginger, Paul G. McKean, Keith Gull

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The flagella connector (FC) of procyclic trypanosomes is a mobile, transmembrane junction important in providing cytotactic morphogenetic information to the daughter cell. Quantitative analyses of FC positioning along the old flagellum, involving direct observations and use of the MPM2 anti-phosphoprotein monoclonal reveals a 'stop point' is reached on the old flagellum which correlates well with the initiation of basal body migration and kinetoplast segregation. This demonstrates further complexeties of the FC and its movement in morphogenetic events in trypanosomes than have hitherto been described. We used intrallagellar transport RNAi mutants to ablate the formation of a new flagellum. Intriguingly the FC could still move, indicating that a motor function beyond the new flagellum is sufficient to move it. When such a FC moves, it drags a sleeve of new flagellar membrane out of the flagellar pocket. This axoneme-less flagellar membrane maintains appropriate developmental relationships to the cell body including following the correct helical path and being connected to the internal cytoskeleton by macula adherens junctions. Movement of the FC in the apparent absence of intraflagellar transport raises the possibility of a new form of motility within a eukaryotic flagellum.

LanguageEnglish
Pages3935-3943
Number of pages9
JournalJournal of Cell Science
Volume119
Issue number19
DOIs
Publication statusPublished - 1 Oct 2006
Externally publishedYes

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Trypanosomiasis
Flagella
Membranes
Basal Bodies
Axoneme
Adherens Junctions
Phosphoproteins
RNA Interference
Cytoskeleton

Cite this

Davidge, Jacqueline A. ; Chambers, Emma ; Dickinson, Harriet A. ; Towers, Katie ; Ginger, Michael L. ; McKean, Paul G. ; Gull, Keith. / Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation. In: Journal of Cell Science. 2006 ; Vol. 119, No. 19. pp. 3935-3943.
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Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagellar membrane formation. / Davidge, Jacqueline A.; Chambers, Emma; Dickinson, Harriet A.; Towers, Katie; Ginger, Michael L.; McKean, Paul G.; Gull, Keith.

In: Journal of Cell Science, Vol. 119, No. 19, 01.10.2006, p. 3935-3943.

Research output: Contribution to journalArticle

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