Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells

Bowie P. Lam; Ka Chi Sarah Cheung; Yun Wah Lam; Stella W. Pang

doi:10.1109/MEMS46641.2020.9056387

Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells

Bowie P. Lam, Ka Chi Sarah Cheung, Yun Wah Lam, Stella W. Pang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

A two-layer scaffold platform was designed for drug screening without using fluorescence staining to examine the effectiveness of the tested compounds. Nasopharyngeal epithelial (NP460) cells were used in this work. Typically, NP460 cells could not squeeze into the 10 mu mathrm{m} narrow trenches but preferred to migrate on the top ridges. However, NP460 cells could migrate into 10 mu mathrm{m} wide trenches after being stimulated by transforming growth factor beta-1 (text{TGF}-beta 1) as the cells became more active. This double-layer scaffold platform served as a drug screening device by tracking the number of cells migrated into narrow trenches after drug treatment to test and monitor the effectiveness of a compound in a biomimetic microenvironment.

Original language	English
Title of host publication	33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	412-415
Number of pages	4
ISBN (Electronic)	9781728135809, 9781728135816
ISBN (Print)	9781728135823
DOIs	https://doi.org/10.1109/MEMS46641.2020.9056387
Publication status	Published - 6 Apr 2020
Externally published	Yes
Event	33rd IEEE International Conference on Micro Electro Mechanical Systems - Vancouver, Canada Duration: 18 Jan 2020 → 22 Jan 2020 Conference number: 33

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Publisher	IEEE
Volume	2020-January
ISSN (Print)	1084-6999
ISSN (Electronic)	2160-1968

Conference

Conference	33rd IEEE International Conference on Micro Electro Mechanical Systems
Abbreviated title	MEMS 2020
Country/Territory	Canada
City	Vancouver
Period	18/01/20 → 22/01/20

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/MEMS46641.2020.9056387Licence: Unspecified

Cite this

Lam, B. P., Cheung, K. C. S., Lam, Y. W., & Pang, S. W. (2020). Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 (pp. 412-415). [9056387] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS46641.2020.9056387

Lam, Bowie P. ; Cheung, Ka Chi Sarah ; Lam, Yun Wah et al. / Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells. 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. pp. 412-415 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

@inproceedings{4e4deccb76c84ea28d722f482100c3c5,

title = "Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells",

abstract = "A two-layer scaffold platform was designed for drug screening without using fluorescence staining to examine the effectiveness of the tested compounds. Nasopharyngeal epithelial (NP460) cells were used in this work. Typically, NP460 cells could not squeeze into the 10 mu mathrm{m} narrow trenches but preferred to migrate on the top ridges. However, NP460 cells could migrate into 10 mu mathrm{m} wide trenches after being stimulated by transforming growth factor beta-1 (text{TGF}-beta 1) as the cells became more active. This double-layer scaffold platform served as a drug screening device by tracking the number of cells migrated into narrow trenches after drug treatment to test and monitor the effectiveness of a compound in a biomimetic microenvironment.",

keywords = "3D Scaffold Platform, Biomimetic, Cell Migration, Drug Screening, NP460 Cells",

author = "Lam, {Bowie P.} and Cheung, {Ka Chi Sarah} and Lam, {Yun Wah} and Pang, {Stella W.}",

note = "Funding Information: This project was supported by the CBNN of City University of Hong Kong (9360148, 9380062) and the University Grants Council of Hong Kong (GRF Projects: 11247716, 11218017, 11213018, and CRF project: C1013-15G). Publisher Copyright: {\textcopyright} 2020 IEEE.; 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 ; Conference date: 18-01-2020 Through 22-01-2020",

year = "2020",

month = apr,

day = "6",

doi = "10.1109/MEMS46641.2020.9056387",

language = "English",

isbn = "9781728135823",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "412--415",

booktitle = "33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020",

address = "United States",

}

Lam, BP, Cheung, KCS, Lam, YW & Pang, SW 2020, Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells. in 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020., 9056387, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2020-January, Institute of Electrical and Electronics Engineers Inc., pp. 412-415, 33rd IEEE International Conference on Micro Electro Mechanical Systems, Vancouver, British Columbia, Canada, 18/01/20. https://doi.org/10.1109/MEMS46641.2020.9056387

Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells. / Lam, Bowie P.; Cheung, Ka Chi Sarah; Lam, Yun Wah et al.

33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., 2020. p. 412-415 9056387 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Cheung, Ka Chi Sarah

AU - Lam, Yun Wah

AU - Pang, Stella W.

N1 - Conference code: 33

PY - 2020/4/6

Y1 - 2020/4/6

N2 - A two-layer scaffold platform was designed for drug screening without using fluorescence staining to examine the effectiveness of the tested compounds. Nasopharyngeal epithelial (NP460) cells were used in this work. Typically, NP460 cells could not squeeze into the 10 mu mathrm{m} narrow trenches but preferred to migrate on the top ridges. However, NP460 cells could migrate into 10 mu mathrm{m} wide trenches after being stimulated by transforming growth factor beta-1 (text{TGF}-beta 1) as the cells became more active. This double-layer scaffold platform served as a drug screening device by tracking the number of cells migrated into narrow trenches after drug treatment to test and monitor the effectiveness of a compound in a biomimetic microenvironment.

AB - A two-layer scaffold platform was designed for drug screening without using fluorescence staining to examine the effectiveness of the tested compounds. Nasopharyngeal epithelial (NP460) cells were used in this work. Typically, NP460 cells could not squeeze into the 10 mu mathrm{m} narrow trenches but preferred to migrate on the top ridges. However, NP460 cells could migrate into 10 mu mathrm{m} wide trenches after being stimulated by transforming growth factor beta-1 (text{TGF}-beta 1) as the cells became more active. This double-layer scaffold platform served as a drug screening device by tracking the number of cells migrated into narrow trenches after drug treatment to test and monitor the effectiveness of a compound in a biomimetic microenvironment.

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Lam BP, Cheung KCS, Lam YW, Pang SW. Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc. 2020. p. 412-415. 9056387. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMS46641.2020.9056387

Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells

Abstract

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UN SDGs

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