Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface

Amy E. Monnington; David J. Cooke

doi:10.1557/opl.2013.343

Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface

Amy E. Monnington, David J. Cooke

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid & glutamic acid-glutamic acid) to the {100} & {111} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the {111} magnetite surface.

Original language	English
Title of host publication	Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications
Editors	Michelle L. Oyen, Shelly R. Peyton, Gila E. Stein
Publisher	Materials Research Society
Pages	239-245
Number of pages	7
Volume	1498
ISBN (Print)	9781605114750, 1605114758
DOIs	https://doi.org/10.1557/opl.2013.343
Publication status	Published - 2013
Event	2012 MRS Fall Meeting - Boston, United States Duration: 25 Nov 2012 → 30 Nov 2012 https://www.mrs.org/fall2012

Publication series

Name	Materials Research Society Symposium Proceedings
Publisher	Materials Research Society
Volume	1498
ISSN (Print)	0272-9172

Conference

Conference	2012 MRS Fall Meeting
Country/Territory	United States
City	Boston
Period	25/11/12 → 30/11/12
Internet address	https://www.mrs.org/fall2012

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10.1557/opl.2013.343Licence: Unspecified

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Monnington, A. E., & Cooke, D. J. (2013). Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. In M. L. Oyen, S. R. Peyton, & G. E. Stein (Eds.), Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications (Vol. 1498, pp. 239-245). (Materials Research Society Symposium Proceedings; Vol. 1498). Materials Research Society. https://doi.org/10.1557/opl.2013.343

Monnington, Amy E. ; Cooke, David J. / Understanding Magnetite Biomineralisation : The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. editor / Michelle L. Oyen ; Shelly R. Peyton ; Gila E. Stein. Vol. 1498 Materials Research Society, 2013. pp. 239-245 (Materials Research Society Symposium Proceedings).

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title = "Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface",

abstract = "Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid & glutamic acid-glutamic acid) to the {100} & {111} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the {111} magnetite surface.",

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Monnington, AE & Cooke, DJ 2013, Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. in ML Oyen, SR Peyton & GE Stein (eds), Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. vol. 1498, Materials Research Society Symposium Proceedings, vol. 1498, Materials Research Society, pp. 239-245, 2012 MRS Fall Meeting, Boston, Massachusetts, United States, 25/11/12. https://doi.org/10.1557/opl.2013.343

Understanding Magnetite Biomineralisation : The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. / Monnington, Amy E.; Cooke, David J.

Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. ed. / Michelle L. Oyen; Shelly R. Peyton; Gila E. Stein. Vol. 1498 Materials Research Society, 2013. p. 239-245 (Materials Research Society Symposium Proceedings; Vol. 1498).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Monnington, Amy E.

AU - Cooke, David J.

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N2 - Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid & glutamic acid-glutamic acid) to the {100} & {111} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the {111} magnetite surface.

AB - Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid & glutamic acid-glutamic acid) to the {100} & {111} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the {111} magnetite surface.

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Monnington AE, Cooke DJ. Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. In Oyen ML, Peyton SR, Stein GE, editors, Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. Vol. 1498. Materials Research Society. 2013. p. 239-245. (Materials Research Society Symposium Proceedings). Epub 2013 Mar 12. doi: 10.1557/opl.2013.343

Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface

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