Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface

Amy E. Monnington; David J. Cooke

doi:10.1557/opl.2013.343

Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface

Amy E. Monnington, David J. Cooke

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid & glutamic acid-glutamic acid) to the {100} & {111} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the {111} magnetite surface.

Original language	English
Title of host publication	Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications
Editors	Michelle L. Oyen, Shelly R. Peyton, Gila E. Stein
Publisher	Materials Research Society
Pages	239-245
Number of pages	7
Volume	1498
ISBN (Print)	9781605114750, 1605114758
DOIs	https://doi.org/10.1557/opl.2013.343
Publication status	Published - 2013
Event	2012 MRS Fall Meeting - Boston, United States Duration: 25 Nov 2012 → 30 Nov 2012 https://www.mrs.org/fall2012

Publication series

Name	Materials Research Society Symposium Proceedings
Publisher	Materials Research Society
Volume	1498
ISSN (Print)	0272-9172

Conference

Conference	2012 MRS Fall Meeting
Country/Territory	United States
City	Boston
Period	25/11/12 → 30/11/12
Internet address	https://www.mrs.org/fall2012

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Monnington, A. E., & Cooke, D. J. (2013). Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. In M. L. Oyen, S. R. Peyton, & G. E. Stein (Eds.), Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications (Vol. 1498, pp. 239-245). (Materials Research Society Symposium Proceedings; Vol. 1498). Materials Research Society. https://doi.org/10.1557/opl.2013.343

Monnington, Amy E. ; Cooke, David J. / Understanding Magnetite Biomineralisation : The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. editor / Michelle L. Oyen ; Shelly R. Peyton ; Gila E. Stein. Vol. 1498 Materials Research Society, 2013. pp. 239-245 (Materials Research Society Symposium Proceedings).

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title = "Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the \{100\} and the \{111\} Surface",

abstract = "Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid \& glutamic acid-glutamic acid) to the \{100\} \& \{111\} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the \{111\} magnetite surface.",

keywords = "Biomaterial, Crystal growth, Fe",

author = "Monnington, \{Amy E.\} and Cooke, \{David J.\}",

year = "2013",

doi = "10.1557/opl.2013.343",

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volume = "1498",

series = "Materials Research Society Symposium Proceedings",

publisher = "Materials Research Society",

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booktitle = "Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications",

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Monnington, AE & Cooke, DJ 2013, Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. in ML Oyen, SR Peyton & GE Stein (eds), Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. vol. 1498, Materials Research Society Symposium Proceedings, vol. 1498, Materials Research Society, pp. 239-245, 2012 MRS Fall Meeting, Boston, Massachusetts, United States, 25/11/12. https://doi.org/10.1557/opl.2013.343

Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. / Monnington, Amy E.; Cooke, David J.
Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. ed. / Michelle L. Oyen; Shelly R. Peyton; Gila E. Stein. Vol. 1498 Materials Research Society, 2013. p. 239-245 (Materials Research Society Symposium Proceedings; Vol. 1498).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Understanding Magnetite Biomineralisation

T2 - 2012 MRS Fall Meeting

AU - Monnington, Amy E.

AU - Cooke, David J.

PY - 2013

Y1 - 2013

N2 - Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid & glutamic acid-glutamic acid) to the {100} & {111} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the {111} magnetite surface.

AB - Magnetite (Fe3O4) formation within Magnetospirillum magneticum strain AMB-1 occurs under the influence of the Mms6 protein. It is hypothesised that if key iron binding sites within the C-terminus of the Mms6 protein are substituted for alanine, the protein's overall iron binding ability is diminished. In this study, an atomistic model of Mms6-driven magnetite formation was developed and the attachment of series amino acid repeats (alanine-alanine, alanine-glutamic acid & glutamic acid-glutamic acid) to the {100} & {111} magnetite surfaces were investigated. Our results suggest the substitution of glutamic acid for alanine residues significantly reduces iron binding affinity of the system, thus confirming the hypothesis. In addition, it is shown that the surface of preferable attachment is the {111} magnetite surface.

KW - Biomaterial

KW - Crystal growth

KW - Fe

UR - https://www.scopus.com/pages/publications/84892404657

U2 - 10.1557/opl.2013.343

DO - 10.1557/opl.2013.343

M3 - Conference contribution

AN - SCOPUS:84892404657

SN - 9781605114750

SN - 1605114758

VL - 1498

T3 - Materials Research Society Symposium Proceedings

SP - 239

EP - 245

BT - Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications

A2 - Oyen, Michelle L.

A2 - Peyton, Shelly R.

A2 - Stein, Gila E.

PB - Materials Research Society

Y2 - 25 November 2012 through 30 November 2012

ER -

Monnington AE, Cooke DJ. Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface. In Oyen ML, Peyton SR, Stein GE, editors, Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications. Vol. 1498. Materials Research Society. 2013. p. 239-245. (Materials Research Society Symposium Proceedings). Epub 2013 Mar 12. doi: 10.1557/opl.2013.343

Understanding Magnetite Biomineralisation: The Effect of Short Amino Acid Sequences on the {100} and the {111} Surface

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