@inproceedings{3ba258f8763140af9745669c165a8922,
title = "UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected",
abstract = "The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 ± 0.4) ml/g and (77.6 ± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w 0, were calculated to be (1.72 ± 0.06) S and (1.77 ± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 ± 1500) g/mol and (40000 ± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change.",
keywords = "Analytical ultracentrifugation, Arabinoxylans, Carboxybenzyl, UV tagging",
author = "Morris, {G. A.} and A. Ebringerova and Harding, {S. E.} and Z. Hromadkova",
year = "1999",
doi = "10.1007/3-540-48703-4_29",
language = "English",
isbn = "9783540661757",
volume = "113",
series = "Progress in Colloid and Polymer Science",
publisher = "Springer Verlag",
pages = "201--204",
editor = "Helmut Colfen",
booktitle = "Analytical Ultracentrifugation V",
address = "Germany",
}