UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected

G. A. Morris, A. Ebringerova, S. E. Harding, Z. Hromadkova

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 ± 0.4) ml/g and (77.6 ± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w 0, were calculated to be (1.72 ± 0.06) S and (1.77 ± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 ± 1500) g/mol and (40000 ± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change.

Original languageEnglish
Title of host publicationAnalytical Ultracentrifugation V
EditorsHelmut Colfen
PublisherSpringer Verlag
Pages201-204
Number of pages4
Volume113
ISBN (Electronic)9783540487036
ISBN (Print)9783540661757
DOIs
Publication statusPublished - 1999
Externally publishedYes

Publication series

NameProgress in Colloid and Polymer Science
PublisherSpringer
Volume113
ISSN (Print)0340-255X

Fingerprint

Xylans
polysaccharides
Structural integrity
Polysaccharides
Sedimentation
integrity
leaves
marking
Viscosity
viscosity
molecular weight
Substitution reactions
Molecular weight
substitutes
corn
mutations
Macromolecules
macromolecules
Hydroxyl Radical
Conformations

Cite this

Morris, G. A., Ebringerova, A., Harding, S. E., & Hromadkova, Z. (1999). UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. In H. Colfen (Ed.), Analytical Ultracentrifugation V (Vol. 113, pp. 201-204). (Progress in Colloid and Polymer Science; Vol. 113). Springer Verlag. Progress in Colloid and Polymer Science https://doi.org/10.1007/3-540-48703-4_29
Morris, G. A. ; Ebringerova, A. ; Harding, S. E. ; Hromadkova, Z. / UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. Analytical Ultracentrifugation V. editor / Helmut Colfen. Vol. 113 Springer Verlag, 1999. pp. 201-204 (Progress in Colloid and Polymer Science). (Progress in Colloid and Polymer Science).
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abstract = "The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 ± 0.4) ml/g and (77.6 ± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w 0, were calculated to be (1.72 ± 0.06) S and (1.77 ± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 ± 1500) g/mol and (40000 ± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change.",
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Morris, GA, Ebringerova, A, Harding, SE & Hromadkova, Z 1999, UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. in H Colfen (ed.), Analytical Ultracentrifugation V. vol. 113, Progress in Colloid and Polymer Science, vol. 113, Springer Verlag, Progress in Colloid and Polymer Science, pp. 201-204. https://doi.org/10.1007/3-540-48703-4_29

UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. / Morris, G. A.; Ebringerova, A.; Harding, S. E.; Hromadkova, Z.

Analytical Ultracentrifugation V. ed. / Helmut Colfen. Vol. 113 Springer Verlag, 1999. p. 201-204 (Progress in Colloid and Polymer Science; Vol. 113), (Progress in Colloid and Polymer Science).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected

AU - Morris, G. A.

AU - Ebringerova, A.

AU - Harding, S. E.

AU - Hromadkova, Z.

PY - 1999

Y1 - 1999

N2 - The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 ± 0.4) ml/g and (77.6 ± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w 0, were calculated to be (1.72 ± 0.06) S and (1.77 ± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 ± 1500) g/mol and (40000 ± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change.

AB - The ultracentrifuge is a useful tool for probing the effects of chemical or other types of substitution/mutation in macromolecules, although very few studies have been performed on polysaccharides. In this study we demonstrate that the substitution of hydroxyl groups on the polysaccharide xylan by p-carboxy-benzyl (CB) bromide groups has little observed effect on molecular weight and little apparent effect on conformation (as monitored by sedimentation velocity and intrinsic viscosity). Corn-cob arabino-(4-O-methylglucurono)xylan (AGX) and its chemically modified derivative CB-AGX were found to have intrinsic viscosities of (82.8 ± 0.4) ml/g and (77.6 ± 0.5) ml/g, respectively. Sedimentation coefficients, s20,w 0, were calculated to be (1.72 ± 0.06) S and (1.77 ± 0.06) S and (weight-average) molecular weights, Mw, from sedimentation equilibrium were found to be (37000 ± 1500) g/mol and (40000 ± 3000) g/mol. From these results we concluded that the UV tagging of arabinoxylan AGX does not significantly change the structural integrity of the xylan molecule; however, the decrease in intrinsic viscosity may indicate a very slight conformational change.

KW - Analytical ultracentrifugation

KW - Arabinoxylans

KW - Carboxybenzyl

KW - UV tagging

UR - http://www.scopus.com/inward/record.url?scp=0008452162&partnerID=8YFLogxK

U2 - 10.1007/3-540-48703-4_29

DO - 10.1007/3-540-48703-4_29

M3 - Conference contribution

SN - 9783540661757

VL - 113

T3 - Progress in Colloid and Polymer Science

SP - 201

EP - 204

BT - Analytical Ultracentrifugation V

A2 - Colfen, Helmut

PB - Springer Verlag

ER -

Morris GA, Ebringerova A, Harding SE, Hromadkova Z. UV tagging leaves the structural integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected. In Colfen H, editor, Analytical Ultracentrifugation V. Vol. 113. Springer Verlag. 1999. p. 201-204. (Progress in Colloid and Polymer Science). (Progress in Colloid and Polymer Science). https://doi.org/10.1007/3-540-48703-4_29