Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins

Jane Harmer, Xin Qi, Gabriella Toniolo, Aysha Patel, Hannah Shaw, Fiona E. Benson, Michael L. Ginger, Paul G. McKean

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

TOF-LisH-PLL motifs define FOP family proteins; some members are involved in flagellum assembly. The critical role of FOP family protein FOR20 is poorly understood. Here, we report relative localisations of the four FOP family proteins in parasitic Trypanosoma brucei: TbRP2, TbOFD1 and TbFOP/FOP1-like are mature basal body proteins whereas TbFOR20 is present on pro- and mature basal bodies – on the latter it localises distal to TbRP2. We discuss how the data, together with published work for another protist Giardia intestinalis, informs on likely FOR20 function. Moreover, our localisation study provides convincing evidence that the antigen recognised by monoclonal antibody YL1/2 at trypanosome mature basal bodies is FOP family protein TbRP2, not tyrosinated α-tubulin as widely stated in the literature. Curiously, FOR20 proteins from T. brucei and closely related African trypanosomes possess short, negatively-charged N-terminal extensions absent from FOR20 in other trypanosomatids and other eukaryotes. The extension is necessary for protein targeting, but insufficient to re-direct TbRP2 to probasal bodies. Yet, FOR20 from the American trypanosome T. cruzi, which lacks any extension, localises to pro- and mature basal bodies when expressed in T. brucei. This identifies unexpected variation in FOR20 architecture that is presently unique to one clade of trypanosomatids.

Original languageEnglish
Pages (from-to)452-466
Number of pages15
JournalProtist
Volume168
Issue number4
DOIs
Publication statusPublished - 1 Aug 2017

Fingerprint

Basal Bodies
Trypanosomiasis
Trypanosoma brucei brucei
Proteins
Giardia lamblia
Flagella
Protein Transport
Tubulin
Eukaryota
Monoclonal Antibodies
Antigens

Cite this

Harmer, J., Qi, X., Toniolo, G., Patel, A., Shaw, H., Benson, F. E., ... McKean, P. G. (2017). Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins. Protist, 168(4), 452-466. https://doi.org/10.1016/j.protis.2017.07.002
Harmer, Jane ; Qi, Xin ; Toniolo, Gabriella ; Patel, Aysha ; Shaw, Hannah ; Benson, Fiona E. ; Ginger, Michael L. ; McKean, Paul G. / Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins. In: Protist. 2017 ; Vol. 168, No. 4. pp. 452-466.
@article{618654eff7a846f380a40311c044a096,
title = "Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins",
abstract = "TOF-LisH-PLL motifs define FOP family proteins; some members are involved in flagellum assembly. The critical role of FOP family protein FOR20 is poorly understood. Here, we report relative localisations of the four FOP family proteins in parasitic Trypanosoma brucei: TbRP2, TbOFD1 and TbFOP/FOP1-like are mature basal body proteins whereas TbFOR20 is present on pro- and mature basal bodies – on the latter it localises distal to TbRP2. We discuss how the data, together with published work for another protist Giardia intestinalis, informs on likely FOR20 function. Moreover, our localisation study provides convincing evidence that the antigen recognised by monoclonal antibody YL1/2 at trypanosome mature basal bodies is FOP family protein TbRP2, not tyrosinated α-tubulin as widely stated in the literature. Curiously, FOR20 proteins from T. brucei and closely related African trypanosomes possess short, negatively-charged N-terminal extensions absent from FOR20 in other trypanosomatids and other eukaryotes. The extension is necessary for protein targeting, but insufficient to re-direct TbRP2 to probasal bodies. Yet, FOR20 from the American trypanosome T. cruzi, which lacks any extension, localises to pro- and mature basal bodies when expressed in T. brucei. This identifies unexpected variation in FOR20 architecture that is presently unique to one clade of trypanosomatids.",
keywords = "Basal body, ciliogenesis, FOP, protein targeting, Trypanosoma brucei, YL1/2.",
author = "Jane Harmer and Xin Qi and Gabriella Toniolo and Aysha Patel and Hannah Shaw and Benson, {Fiona E.} and Ginger, {Michael L.} and McKean, {Paul G.}",
year = "2017",
month = "8",
day = "1",
doi = "10.1016/j.protis.2017.07.002",
language = "English",
volume = "168",
pages = "452--466",
journal = "Protist",
issn = "1434-4610",
publisher = "Urban und Fischer Verlag Jena",
number = "4",

}

Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins. / Harmer, Jane; Qi, Xin; Toniolo, Gabriella; Patel, Aysha; Shaw, Hannah; Benson, Fiona E.; Ginger, Michael L.; McKean, Paul G.

In: Protist, Vol. 168, No. 4, 01.08.2017, p. 452-466.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Variation in Basal Body Localisation and Targeting of Trypanosome RP2 and FOR20 Proteins

AU - Harmer, Jane

AU - Qi, Xin

AU - Toniolo, Gabriella

AU - Patel, Aysha

AU - Shaw, Hannah

AU - Benson, Fiona E.

AU - Ginger, Michael L.

AU - McKean, Paul G.

PY - 2017/8/1

Y1 - 2017/8/1

N2 - TOF-LisH-PLL motifs define FOP family proteins; some members are involved in flagellum assembly. The critical role of FOP family protein FOR20 is poorly understood. Here, we report relative localisations of the four FOP family proteins in parasitic Trypanosoma brucei: TbRP2, TbOFD1 and TbFOP/FOP1-like are mature basal body proteins whereas TbFOR20 is present on pro- and mature basal bodies – on the latter it localises distal to TbRP2. We discuss how the data, together with published work for another protist Giardia intestinalis, informs on likely FOR20 function. Moreover, our localisation study provides convincing evidence that the antigen recognised by monoclonal antibody YL1/2 at trypanosome mature basal bodies is FOP family protein TbRP2, not tyrosinated α-tubulin as widely stated in the literature. Curiously, FOR20 proteins from T. brucei and closely related African trypanosomes possess short, negatively-charged N-terminal extensions absent from FOR20 in other trypanosomatids and other eukaryotes. The extension is necessary for protein targeting, but insufficient to re-direct TbRP2 to probasal bodies. Yet, FOR20 from the American trypanosome T. cruzi, which lacks any extension, localises to pro- and mature basal bodies when expressed in T. brucei. This identifies unexpected variation in FOR20 architecture that is presently unique to one clade of trypanosomatids.

AB - TOF-LisH-PLL motifs define FOP family proteins; some members are involved in flagellum assembly. The critical role of FOP family protein FOR20 is poorly understood. Here, we report relative localisations of the four FOP family proteins in parasitic Trypanosoma brucei: TbRP2, TbOFD1 and TbFOP/FOP1-like are mature basal body proteins whereas TbFOR20 is present on pro- and mature basal bodies – on the latter it localises distal to TbRP2. We discuss how the data, together with published work for another protist Giardia intestinalis, informs on likely FOR20 function. Moreover, our localisation study provides convincing evidence that the antigen recognised by monoclonal antibody YL1/2 at trypanosome mature basal bodies is FOP family protein TbRP2, not tyrosinated α-tubulin as widely stated in the literature. Curiously, FOR20 proteins from T. brucei and closely related African trypanosomes possess short, negatively-charged N-terminal extensions absent from FOR20 in other trypanosomatids and other eukaryotes. The extension is necessary for protein targeting, but insufficient to re-direct TbRP2 to probasal bodies. Yet, FOR20 from the American trypanosome T. cruzi, which lacks any extension, localises to pro- and mature basal bodies when expressed in T. brucei. This identifies unexpected variation in FOR20 architecture that is presently unique to one clade of trypanosomatids.

KW - Basal body

KW - ciliogenesis

KW - FOP

KW - protein targeting

KW - Trypanosoma brucei

KW - YL1/2.

UR - http://www.scopus.com/inward/record.url?scp=85027493758&partnerID=8YFLogxK

U2 - 10.1016/j.protis.2017.07.002

DO - 10.1016/j.protis.2017.07.002

M3 - Article

VL - 168

SP - 452

EP - 466

JO - Protist

JF - Protist

SN - 1434-4610

IS - 4

ER -