Weak self-association in a carbohydrate system

Trushar R. Patel, Stephen E. Harding, Anna Ebringerova, Marcin Deszczynski, Zdenka Hromadkova, Adiaratou Togola, Berit Smestad Paulsen, Gordon A. Morris, Arthur J. Rowe

Research output: Contribution to journalArticle

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Abstract

The physiological importance of weak interactions between biological macromolecules (molar dissociation constants >10 μM) is now well recognized, particularly with regard to cell adhesion and immunological phenomena, and many weak interactions have been measured for proteins. The concomitant importance of carbohydrate-carbohydrate interactions has also been identified, although no weak interaction between pure carbohydrate systems has ever been measured. We now demonstrate for the first time to our knowledge using a powerful probe for weak interactions - sedimentation velocity in the analytical ultracentrifuge - that at least some carbohydrates (from the class of polysaccharides known as heteroxylans and demonstrated here to be biologically active) can show well-defined weak self-interactions of the "monomer- dimer" type frequently found in protein systems. The weak interaction between the heteroxylans is shown from a temperature dependence study to be likely to be hydrophobic in nature.

LanguageEnglish
Pages741-749
Number of pages9
JournalBiophysical Journal
Volume93
Issue number3
DOIs
Publication statusPublished - Aug 2007
Externally publishedYes

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Carbohydrates
Cell Adhesion
Polysaccharides
Proteins
Temperature

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Patel, T. R., Harding, S. E., Ebringerova, A., Deszczynski, M., Hromadkova, Z., Togola, A., ... Rowe, A. J. (2007). Weak self-association in a carbohydrate system. Biophysical Journal, 93(3), 741-749. https://doi.org/10.1529/biophysj.106.100891
Patel, Trushar R. ; Harding, Stephen E. ; Ebringerova, Anna ; Deszczynski, Marcin ; Hromadkova, Zdenka ; Togola, Adiaratou ; Paulsen, Berit Smestad ; Morris, Gordon A. ; Rowe, Arthur J. / Weak self-association in a carbohydrate system. In: Biophysical Journal. 2007 ; Vol. 93, No. 3. pp. 741-749.
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Patel, TR, Harding, SE, Ebringerova, A, Deszczynski, M, Hromadkova, Z, Togola, A, Paulsen, BS, Morris, GA & Rowe, AJ 2007, 'Weak self-association in a carbohydrate system', Biophysical Journal, vol. 93, no. 3, pp. 741-749. https://doi.org/10.1529/biophysj.106.100891

Weak self-association in a carbohydrate system. / Patel, Trushar R.; Harding, Stephen E.; Ebringerova, Anna; Deszczynski, Marcin; Hromadkova, Zdenka; Togola, Adiaratou; Paulsen, Berit Smestad; Morris, Gordon A.; Rowe, Arthur J.

In: Biophysical Journal, Vol. 93, No. 3, 08.2007, p. 741-749.

Research output: Contribution to journalArticle

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Patel TR, Harding SE, Ebringerova A, Deszczynski M, Hromadkova Z, Togola A et al. Weak self-association in a carbohydrate system. Biophysical Journal. 2007 Aug;93(3):741-749. https://doi.org/10.1529/biophysj.106.100891