Whey protein augments leucinemia and postexercise p70s6k1 activity compared with a hydrolyzed collagen blend when in recovery from training with low carbohydrate availability

Samuel Impey, Kelly Hammond, Robert Naughton, Carl Langan-Evans, Sam Shepherd, Adam Sharples, Kenneth Smith, Stewart Jeromson, David Lee Hamilton, Graeme Close, James Morton

Research output: Contribution to journalArticle

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Abstract

We examined the effects of whey versus collagen protein on skeletal muscle cell signalling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate (CHO) availability. In a repeated measures design (after adhering to a 36-h exercise-dietary intervention to standardise pre-exercise muscle glycogen), eight males completed a 75-min non-exhaustive cycling protocol and consumed 22 g of a hydrolysed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise and 22 g immediately post-exercise. Exercise decreased (P<0.05) muscle glycogen content by comparable levels from pre-to post-exercise in both trials (≈ 300 to 150 mmol.kg-1 dw). WHEY protein induced greater increases in plasma BCAAs (P=0.03) and leucine (P=0.02) than COLLAGEN. Exercise induced (P<0.05) similar increases in PGC-1α (5-fold) mRNA at 1.5 h post-exercise between conditions though no affect of exercise (P>0.05) was observed for p53, Parkin and Beclin1 mRNA. Exercise suppressed (P<0.05) p70S6K1 activity in both conditions immediately post-exercise (≈ 25 fmol.min-1.mg-1). Post-exercise feeding increased p70S6K1 activity at 1.5 h post-exercise (P<0.05), the magnitude of which was greater (P <0.05) in WHEY (180 ± 105 fmol.min-1.mg-1) versus COLLAGEN (73 ± 42 fmol.min-1.mg-1). We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low CHO availability. In contrast, whey protein augments post-exercise p70S6K activity compared with hydrolysed collagen, as likely mediated via increased leucine availability.
LanguageEnglish
Pages651-659
Number of pages9
JournalInternational Journal of Sport Nutrition and Exercise Metabolism
Volume28
Issue number6
Early online dateMay 2018
DOIs
Publication statusPublished - 1 Nov 2018

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Collagen
Carbohydrates
Organelle Biogenesis
70-kDa Ribosomal Protein S6 Kinases
Mitochondrial Proteins
Glycogen
Leucine
Muscle Cells
Skeletal Muscle
Proteins
Muscles
Messenger RNA
Whey Proteins
Beclin-1
Whey

Cite this

Impey, Samuel ; Hammond, Kelly ; Naughton, Robert ; Langan-Evans, Carl ; Shepherd, Sam ; Sharples, Adam ; Smith, Kenneth ; Jeromson, Stewart ; Hamilton, David Lee ; Close, Graeme ; Morton, James. / Whey protein augments leucinemia and postexercise p70s6k1 activity compared with a hydrolyzed collagen blend when in recovery from training with low carbohydrate availability. In: International Journal of Sport Nutrition and Exercise Metabolism. 2018 ; Vol. 28, No. 6. pp. 651-659.
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Whey protein augments leucinemia and postexercise p70s6k1 activity compared with a hydrolyzed collagen blend when in recovery from training with low carbohydrate availability. / Impey, Samuel ; Hammond, Kelly; Naughton, Robert; Langan-Evans, Carl; Shepherd, Sam; Sharples, Adam; Smith, Kenneth ; Jeromson, Stewart; Hamilton, David Lee; Close, Graeme; Morton, James.

In: International Journal of Sport Nutrition and Exercise Metabolism, Vol. 28, No. 6, 01.11.2018, p. 651-659.

Research output: Contribution to journalArticle

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T1 - Whey protein augments leucinemia and postexercise p70s6k1 activity compared with a hydrolyzed collagen blend when in recovery from training with low carbohydrate availability

AU - Impey, Samuel

AU - Hammond, Kelly

AU - Naughton, Robert

AU - Langan-Evans, Carl

AU - Shepherd, Sam

AU - Sharples, Adam

AU - Smith, Kenneth

AU - Jeromson, Stewart

AU - Hamilton, David Lee

AU - Close, Graeme

AU - Morton, James

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AB - We examined the effects of whey versus collagen protein on skeletal muscle cell signalling responses associated with mitochondrial biogenesis and protein synthesis in recovery from an acute training session completed with low carbohydrate (CHO) availability. In a repeated measures design (after adhering to a 36-h exercise-dietary intervention to standardise pre-exercise muscle glycogen), eight males completed a 75-min non-exhaustive cycling protocol and consumed 22 g of a hydrolysed collagen blend (COLLAGEN) or whey (WHEY) protein 45 min prior to exercise, 22 g during exercise and 22 g immediately post-exercise. Exercise decreased (P<0.05) muscle glycogen content by comparable levels from pre-to post-exercise in both trials (≈ 300 to 150 mmol.kg-1 dw). WHEY protein induced greater increases in plasma BCAAs (P=0.03) and leucine (P=0.02) than COLLAGEN. Exercise induced (P<0.05) similar increases in PGC-1α (5-fold) mRNA at 1.5 h post-exercise between conditions though no affect of exercise (P>0.05) was observed for p53, Parkin and Beclin1 mRNA. Exercise suppressed (P<0.05) p70S6K1 activity in both conditions immediately post-exercise (≈ 25 fmol.min-1.mg-1). Post-exercise feeding increased p70S6K1 activity at 1.5 h post-exercise (P<0.05), the magnitude of which was greater (P <0.05) in WHEY (180 ± 105 fmol.min-1.mg-1) versus COLLAGEN (73 ± 42 fmol.min-1.mg-1). We conclude that protein composition does not modulate markers of mitochondrial biogenesis when in recovery from a training session deliberately completed with low CHO availability. In contrast, whey protein augments post-exercise p70S6K activity compared with hydrolysed collagen, as likely mediated via increased leucine availability.

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