Throughout forensic research into stabbing incidents, the majority of the focus has been placed on the physical wound on the body. However, this approach is not suitable for all scenarios as the medical care of the victim has to be considered as the number one priority if they are still alive once first responders arrive on scene. Therefore, it is necessary for forensic research to pivot to determine damage characteristics from something other than the wound on the body. This information can also be determined from the clothing the knife passed through. However, due to the lack of focus on this area of research, no standardised methodology exists that measures all aspects of damage and the characteristics of the blade that may impact the damage, particularly the sharpness of the blade edge. This research, therefore, focused on determining an experimental protocol that contained all of the required data points, allowing a full profile of the damage to the fabric to be obtained. The experimental protocol began by testing the sharpness of the blade edge at varying distances from the tip of the blade. The sharpness was determined in sequence with the stab damage generation to create a link between the sharpness of the blade edge and the generated damage that has not been shown previously in research. The stab damage was then generated for each fabric using two different orientations of the fabric and two different backing materials (polystyrene and no backing material). The generated damage was then imaged to allow for measurement of specific damage characteristics, including the length of the damage and the distortion of the yarns. Further trends of characteristics were determined by categorising different levels of damage using the methodology determined by Bostock et al. (2013). The experimental methodology was completed using a repurposed 3D printer, which allowed a knife to be moved on its z-axis. This machine was used to complete both the sharpness testing and the stab damage generation for the entirety of the research, as the device allowed the programming of the movement, ensuring that the reproducibility and repeatability of the data collection was high. 3D prints were also used throughout the research to facilitate the attachment of the knife to the 3D printer for all of the data collection. They were also used to secure the fabric during the stab damage generation and attach the thread and fabric holders to a loadcell that recorded the sharpness and penetration force of each test. This protocol was evaluated to determine the suitability of each data collection and analysis step for use in a working forensic laboratory for both research and legal purposes. The research also identified trends in damage characteristics among different blade and fabric types that will aid forensic examiners in determining the blade type when the blade is not recovered from the scene of the crime.